MVP Antibody (1032) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-47807

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Flow Cytometry, Immunofluorescence, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1032

Format

Azide and BSA Free
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Product Specifications

Immunogen

proteins precipitated from human breast cancer MCF-7 cells

Localization

Cytoplasmic

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44401).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for MVP Antibody (1032) - Azide and BSA Free

Immunohistochemistry-Paraffin: MVP Antibody (1032) - Azide and BSA Free [NBP2-47807]

Immunohistochemistry-Paraffin: MVP Antibody (1032) - Azide and BSA Free [NBP2-47807]

Immunohistochemistry-Paraffin: MVP Antibody (1032) - Azide and BSA Free [NBP2-47807] - Human Testicular Carcinoma stained with MVP Monoclonal Antibody (1032).
Immunohistochemistry-Paraffin: MVP Antibody (1032) - Azide and BSA Free [NBP2-47807]

Immunohistochemistry-Paraffin: MVP Antibody (1032) - Azide and BSA Free [NBP2-47807]

Immunohistochemistry-Paraffin: MVP Antibody (1032) - Azide and BSA Free [NBP2-47807] - Human Breast Carcinoma stained with MVP Monoclonal Antibody (1032).

Applications for MVP Antibody (1032) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5 - 1 ug/million cells in 0.1 ml

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunofluorescence

0.5 - 1.0 ug/ml

Immunohistochemistry-Paraffin

0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA buffer, pH 7.5-8.5, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

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Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: MVP

MVP encodes the major vault protein which is a lung resistance-related protein. Vaults are multi-subunit structures that may be involved in nucleo-cytoplasmic transport. This protein mediates drug resistance, perhaps via a transport process. It is widely distributed in normal tissues, and overexpressed in multidrug-resistant cancer cells. The protein overexpression is a potentially useful marker of clinical drug resistance. This gene produces two transcripts by using two alternative exon 2 sequences; however, the open reading frames are the same in both transcripts.

Alternate Names

LRPVAULT1, Lung resistance-related protein, major vault protein

Gene Symbol

MVP

Additional MVP Products

Product Documents for MVP Antibody (1032) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for MVP Antibody (1032) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs for MVP Antibody (1032) - Azide and BSA Free

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  • Q: Are there any MVP antibodies that will recognize mouse and can be used for immunofluorescence?

    A: At this time we do not have an MVP antibody that will recognize mouse and has been used for immunofluorescence. If you would like to try an antibody in an untested species or application you would qualify for our Innovator's Reward program. Through this program if you complete an online review with image, detailing your positive or negative results we will send you a discount voucher for 100% of the purchase price of the reviewed product.

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