Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2G12

Format

Azide and BSA Free
View all MxA/Mx1 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human MX1 (NP_002453) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

75.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse MxA/Mx1 Antibody (OTI2G12) - Azide and BSA Free (NBP1-47859) is a monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for MxA/Mx1 Antibody (OTI2G12) - Azide and BSA Free

Western Blot: MxA/Mx1 Antibody (OTI2G12)Azide and BSA Free [NBP2-72838]

Western Blot: MxA/Mx1 Antibody (OTI2G12)Azide and BSA Free [NBP2-72838]

Western Blot: MxA/Mx1 Antibody (OTI2G12) - Azide and BSA Free [NBP2-72838] - MX1 ( Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MX1. Positive lysates.
Immunocytochemistry/ Immunofluorescence: MxA/Mx1 Antibody (OTI2G12) - Azide and BSA Free [NBP2-72838]

Immunocytochemistry/ Immunofluorescence: MxA/Mx1 Antibody (OTI2G12) - Azide and BSA Free [NBP2-72838]

Immunocytochemistry/Immunofluorescence: MxA/Mx1 Antibody (OTI2G12) - Azide and BSA Free [NBP2-72838] - Anti-MX1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MX1.
Western Blot: MxA/Mx1 Antibody (OTI2G12)Azide and BSA Free [NBP2-72838]

Western Blot: MxA/Mx1 Antibody (OTI2G12)Azide and BSA Free [NBP2-72838]

Western Blot: MxA/Mx1 Antibody (OTI2G12) - Azide and BSA Free [NBP2-72838] - Analysis of extracts (10ug) from 1 cell lines by using anti-MX1 monoclonal antibody at 1:200 dilution.
Western Blot: MxA/Mx1 Antibody (OTI2G12)Azide and BSA Free [NBP2-72838]

Western Blot: MxA/Mx1 Antibody (OTI2G12)Azide and BSA Free [NBP2-72838]

Western Blot: MxA/Mx1 Antibody (OTI2G12) - Azide and BSA Free [NBP2-72838] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-MX1 monoclonal antibody (1:200).

Applications for MxA/Mx1 Antibody (OTI2G12) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MxA/Mx1

In mouse, the interferon-inducible Mx protein is responsible for a specific antiviral state against influenza virus infection. The protein encoded by this gene is similar to the mouse protein as determined by its antigenic relatedness, induction conditions, physicochemical properties, and amino acid analysis. This cytoplasmic protein is a member of both the dynamin family and the family of large GTPases. [provided by RefSeq]

Long Name

Interferon-regulated Resistance GTP-binding protein MxA

Alternate Names

IFI-78K, IFI78, Mx1

Gene Symbol

MX1

Additional MxA/Mx1 Products

Product Documents for MxA/Mx1 Antibody (OTI2G12) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for MxA/Mx1 Antibody (OTI2G12) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Infectious Diseases

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Protocols

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FAQs

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