Myc Epitope Tag Antibody (2) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-43627

Novus Biologicals
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Key Product Details

Species Reactivity

Epitope Tag

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2

Format

Azide and BSA Free
View all Myc Epitope Tag Primary Antibodies »

Product Specifications

Immunogen

The immunogen used to generate this antibody corresponds to Myc Epitope Tag

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Myc Epitope Tag Antibody (2) - Azide and BSA Free

Western Blot: Myc Epitope Tag Antibody (2) [NBP2-43627]

Western Blot: Myc Epitope Tag Antibody (2) [NBP2-43627]

Western Blot: Myc Epitope Tag Antibody (2) [NBP2-43627] - Myc tag antibody [0002] Immunoprecipitation of Myc tagged protein. HeLa transfected lysates were subjected to immunoprecipitation using (B) normal mouse IgG or (C) 2.5 ug of anti-Myc tag antibody [0002]. (A) Input, 20ug of HeLa transfected lysates. The precipitated protein was detected. EasyBlot anti-Mouse IgG Kit was used in Western blot.
Immunocytochemistry/ Immunofluorescence: Myc Epitope Tag Antibody (2) [NBP2-43627]

Immunocytochemistry/ Immunofluorescence: Myc Epitope Tag Antibody (2) [NBP2-43627]

Immunocytochemistry/Immunofluorescence: Myc Epitope Tag Antibody (2) [NBP2-43627] - Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Myc tag stained by Myc tag antibody [0002] (NBP2-43627) diluted at 1:2000.
Myc Epitope Tag Antibody (2)

Western Blot: Myc Epitope Tag Antibody (2) [NBP2-43627] -

Western Blot: Myc Epitope Tag Antibody (2) [NBP2-43627] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Myc Epitope Tag antibody [GT0002] (NBP2-43627) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Myc Epitope Tag Antibody (2) - Azide and BSA Free

ELISA: Myc Epitope Tag Antibody (2) [NBP2-43627] -

Indirect ELISA analysis was performed by coating the plate with recombinant Multiple Tags Positive Control ( 6xHis-MBP-DDDDK-V5-HSV-HA-T7-Myc-S) (57.14-0.89 nM). Coated protein was probed with Myc tag antibody [GT0002] (NBP2-43627) (1 ug/mL). Goat antibody Mouse IgG antibody (HRP) (1:10000) was used to detect the bound primary antibody.

Applications for Myc Epitope Tag Antibody (2) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:2000

Immunoprecipitation

1:100-1:500

Western Blot

1:5000-1:20000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Myc Epitope Tag

Alternate Names

BHLHE39, Class E basic helix-loop-helix protein 39 (bHLHe39), Myc proto-oncogene protein, Proto-oncogene c-Myc, Transcription factor p64

Additional Myc Epitope Tag Products

Product Documents for Myc Epitope Tag Antibody (2) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Myc Epitope Tag Antibody (2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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