MyD88 Antibody (4D6) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-80862

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Primate, Rhesus Macaque

Applications

Western Blot, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4D6

Format

Azide and BSA Free
View all MyD88 Primary Antibodies »

Product Specifications

Immunogen

A portion of amino acids 50-100 of human MyD88 was used as the immunogen for this antibody.

Reactivity Notes

Gorilla, Chimp, Orangutan (100%), Rat (63%), Equine (82%), Canine (73%), Cat, Mouse (68%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for MyD88 Antibody (4D6) - Azide and BSA Free

Western Blot: MyD88 Antibody (4D6)Azide and BSA Free [NBP2-80862]

Western Blot: MyD88 Antibody (4D6)Azide and BSA Free [NBP2-80862]

Western Blot: MyD88 Antibody (4D6) - Azide and BSA Free [NBP2-80862] - Normal human bronchial epithelial total cell lysate. Image from verified customer review. Image from the standard format of this antibody.
Immunocytochemistry: MyD88 Antibody (4D6) - Azide and BSA Free [NBP2-80862]

Immunocytochemistry: MyD88 Antibody (4D6) - Azide and BSA Free [NBP2-80862]

Immunocytochemistry: MyD88 Antibody (4D6) - Azide and BSA Free [NBP2-80862] - Analysis of MyD88 antibody in MCF-7 cells using an isotype control (top) and MyD88 Antibody (bottom) at 5 ug/ml. Image from the standard format of this antibody.
Flow Cytometry: MyD88 Antibody (4D6) - Azide and BSA Free [NBP2-80862]

Flow Cytometry: MyD88 Antibody (4D6) - Azide and BSA Free [NBP2-80862]

Flow Cytometry: MyD88 Antibody (4D6) - Azide and BSA Free [NBP2-80862] - Intracellular analysis of MyD88 antibody in Jurkat cells using 0.5 ug. Shaded histogram represents cells without antibody; green represents isotype control; red represents MyD88 antibody. This antibody was used for this test, and an anti-mouse IgG PE conjugated secondary antibody. Image from the standard format of this antibody.
Western Blot: MyD88 Antibody (4D6)Azide and BSA Free [NBP2-80862]

Western Blot: MyD88 Antibody (4D6)Azide and BSA Free [NBP2-80862]

Western Blot: MyD88 Antibody (4D6) - Azide and BSA Free [NBP2-80862] - Analysis of MyD88 in A) human ovary, B) human prostate and Jurkat cell lysate in the C) absence and D) presence of immunizing peptide using this antibody. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test. Image from the standard format of this antibody.
Flow Cytometry: MyD88 Antibody (4D6) - Azide and BSA Free [NBP2-80862]

Flow Cytometry: MyD88 Antibody (4D6) - Azide and BSA Free [NBP2-80862]

Flow Cytometry: MyD88 Antibody (4D6) - Azide and BSA Free [NBP2-80862] - An intracellular stain was performed on MCF7 cells with MyD88 (4D6) antibody NBP2-27369PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to phycoerythrin.
Flow Cytometry: MyD88 Antibody (4D6) - Azide and BSA Free [NBP2-80862]

Flow Cytometry: MyD88 Antibody (4D6) - Azide and BSA Free [NBP2-80862]

Flow Cytometry: MyD88 Antibody (4D6) - Azide and BSA Free [NBP2-80862] - An intracellular stain was performed on MCF7 cells with MyD88 [4D6] Antibody NBP2-27369AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 488.

Applications for MyD88 Antibody (4D6) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5ug/10^6cells

Immunocytochemistry/ Immunofluorescence

5ug/ml

Western Blot

5-7ug/ml
Application Notes
This antibody is Cytof ready.

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  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MyD88

MyD88, a protein involved in interleukin-1 (IL-1) mediated signalling was originally isolated as myeloid differentiation primary response gene (3,4). MyD88 possesses a N-terminal death domain similar to cytoplasmic segments of TNF receptor 1, Fas, and C-terminal region related to IL-1 and Toll receptors. Overexpression of MyD88 induces activation the c-Jun N-terminal kinase and NF-kB through its death domain (1,2). The C-terminus of MyD88 interacts with the IL-1 receptor and blocks NF-kB activation induced by IL-1, but not by NF-kB.

Long Name

Myeloid Differentiation Primary Response Gene 88

Alternate Names

MYD88D, myeloid differentiation primary response gene (88), myeloid differentiation primary response protein MyD88

Gene Symbol

MYD88

Additional MyD88 Products

Product Documents for MyD88 Antibody (4D6) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for MyD88 Antibody (4D6) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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