MYH6 Antibody (3-48) - Azide and BSA Free

Novus Biologicals | Catalog # NB300-284

Novus Biologicals
Discontinued Product
NB300-284 has been discontinued. View all MYH6 products.

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine

Cited:

Mouse, Rat, Canine

Predicted:

Bovine (97%), Rabbit (91%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3-48

Format

Azide and BSA Free
View all MYH6 Primary Antibodies »

Product Specifications

Immunogen

Human ventricular myosin

Reactivity Notes

Canine reactivity reported in (PMID: 23577316). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions. Predicted to react with Rabbit, Bovine.

Specificity

This antibody recognizes alpha and beta type heavy chains of light meromyosin fragments (LMM) of cardiac myosin heavy chains. It also reacts with LMM fragments of cardiac myosin and beta heavy chain of slow human skeletal muscle. The antibody does not crossreact with human myosin light chains.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for MYH6 Antibody (3-48) - Azide and BSA Free

Western Blot: MYH6 Antibody (3-48)Azide and BSA Free [NB300-284]

Western Blot: MYH6 Antibody (3-48)Azide and BSA Free [NB300-284]

Western Blot: MYH6 Antibody (3-48) [NB300-284] - analysis of total cardiac myosin expression in tissue lysates of rat heart (lanes 1-5), liver (lanes 6-7), and lung (lanes 8-9). Increasing amounts of total protein were loaded as follow: lanes 1, 6 and 8: 0 ug; lane 2: 50 ug; lanes 3, 7 and 9: 250 ug; lane 5: 500 ug. (anti-cMHC was used at 1:500; secondary antibody: Goat anti-mouse IgG Fc-HRP; 1:5000)
Immunohistochemistry-Paraffin: MYH6 Antibody (3-48) - Azide and BSA Free [NB300-284]

Immunohistochemistry-Paraffin: MYH6 Antibody (3-48) - Azide and BSA Free [NB300-284]

Immunohistochemistry-Paraffin: MYH6 Antibody (3-48) [NB300-284] - Formaldehyde-fixed paraffin-embedded (FFPE) Tissue Slides. Human heart ventricle following immunostaining with cMHC 3-48 and irrelevant NeuAc monoclonal antibody at low microscopical magnification.
Western Blot: MYH6 Antibody (3-48)Azide and BSA Free [NB300-284]

Western Blot: MYH6 Antibody (3-48)Azide and BSA Free [NB300-284]

MYH6-Antibody-3-48-Western-Blot-NB300-284-img0006.jpg
MYH6 Antibody (3-48) - Azide and BSA Free

Western Blot: MYH6 Antibody (3-48) - Azide and BSA Free [NB300-284] -

Western blot immunodetection of cardiac MyHC isoforms from left ventricle (LV) homogenates and extracts of adult euthyroid (EU), hypothyroid (HY), and hyperthyroid (TH) Lewis rats. The membranes were stained by NB300-284 antibody recognizing both MyHC alpha and MyHC beta isoforms (a) or by BA.G5 antibody, which was solely specific for cardiac MyHC alpha isoform (b).
MYH6 Antibody (3-48) - Azide and BSA Free

Western Blot: MYH6 Antibody (3-48) - Azide and BSA Free [NB300-284] -

Western Blot: MYH6 Antibody (3-48) - Azide and BSA Free [NB300-284] - Western blot immunodetection of cardiac MyHC isoforms from left ventricle (LV) homogenates & extracts of adult euthyroid (EU), hypothyroid (HY), & hyperthyroid (TH) Lewis rats. The membranes were stained by NB300-284 antibody recognizing both MyHC alpha & MyHC beta isoforms (a) or by BA.G5 antibody, which was solely specific for cardiac MyHC alpha isoform (b). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/22187528), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for MYH6 Antibody (3-48) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:300

Immunohistochemistry-Paraffin

1:300

Western Blot

1:500
Application Notes
Please only use IgG speciific secondaries with this product. Fab region specific secondaries may not detect this antibody. ICC/IF reactivity reported in (PMID: 23577316).

Reviewed Applications

Read 2 reviews rated 4.5 using NB300-284 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Lyophilized protein G purified in PBS pH7.4 (Full IgG)

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MYH6

Myosin is a highly conserved, ubiquitously expressed protein that hydrolyzes ATP, and this reaction provides the energy required for muscle contraction and that interacts with Actin to generate the force for cellular movements. It is a hexameric protein composed of four light chains and two heavy chains, each containing an actin-binding site and an ATP hydrolysis site. The heavy chains are encoded by the MYH gene family and were first isolated from a human fetal skeletal muscle and are the major determinant in the speed of contraction of skeletal muscle. Cardiac myosin exists as two isoforms in humans, a-cardiac myosins and b-cardiac myosins. These two isoforms are expressed in different amounts in the human heart. b-cardiac myosins is the predominant form during normal physiology while the a-isoform contributes for approximately 7% of the total myosin. Mutations of the MYH genes are associated with several different dilated and hypertrophic cardiomyopathies.

Long Name

Myosin Heavy Chain 6, Cardiac Muscle, Alpha

Alternate Names

alpha-MHC, ASD3, CMD1EE, CMH14, MyHC-Alpha, MYHCA, Myosin-6, SSS3

Entrez Gene IDs

79784 (Human); 71960 (Mouse); 308572 (Rat)

Gene Symbol

MYH6

UniProt

Q7Z406 (Human)

Additional MYH6 Products

Product Documents for MYH6 Antibody (3-48) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for MYH6 Antibody (3-48) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cardiac Stem Cell Markers

Citations for MYH6 Antibody (3-48) - Azide and BSA Free

Customer Reviews for MYH6 Antibody (3-48) - Azide and BSA Free (2)

4.5 out of 5
2 Customer Ratings
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Customer Images

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested:
    Species: Human
    Verified Customer | Posted 11/25/2014
    MYH6 Antibody (3-48) - Azide and BSA Free NB300-284
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Rat
    Species: Rat
    Verified Customer | Posted 12/30/2011

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Protocols

View specific protocols for MYH6 Antibody (3-48) - Azide and BSA Free (NB300-284):

Immunohistochemistry-Paraffin Protocol Specific for NB300-284: Myosin heavy chain Antibody (3-48):
Materials

1) 1 Phosphate buffered saline (pH 7.6): NaCl 137mmol/L, KCl 2.7mmol/L, Na2HPO4 4.3mmol/L, KH2PO4 1.4 mmol/L
2) Citrate buffer, 0.01 M, pH6.0, Sodium Citrate 3g, Citric acid 0.4g
3) 3% Hydrogen peroxide
4) Primary antibody
5) Blocking serum (normal serum)
6) Biotinylated secondary antibody
7) DAB staining kit

Methods

1. Dewax and hydration of slides using xylene and EtOH:
Dry slides for 20 min in a 60 C oven
Add Xylene, 2 x 10 min
100%, 95%, 80%, and 70% EtOH, 5 min each EtOH concentration
Rinse in PBS, 5'

2 Antigen retrieval method (only for paraffin slides)
1a. High-pressure antigen retrieval procedure (recommended method)
Place slides in a glass slide holder (ensure that the slide holder is completely filled with slides, slides without sections if necessary, to ensure even heating. The entire slide holder is immersed in 1000 ml of Citrate buffer (0.01M, pH6.0) within a pressure cooker
Once steam is produced, and ONLY when steam is visible, from the pressure cooker (usually 15-20 min), the required high-pressure will have been reached, and slides will be incubated for 2 min.
Turn off heat, and allow buffer and slides to cool to room temperature
Slides are then rinsed in PBS for 5 minutes
2. Add 3% hydrogen peroxide solution, 10'at RT, then PBS, 3X5'
3. Normal blocking serum, 20'at RT
4. Incubate with Primary Ab, 4C overnight or 1.5 hours at 37C
5. Rinse with PBS, 3 X 5' each rinse
6. Add Biotin-conjugated second antibody, 10'at RT
7. Rinse with PBS, 3 X 5' each rinse
8. Add Streptavidin-Peroxidase, 10'at RT
9. Rinse with PBS, 3 X 5' each rinse
10. Staining with DAB solution, 2-5'under microscope
11. Stop the reaction by washing in tap water
12. Counterstain in Haematoxylin for 3-5 minutes
13. 75%, 80%, 95% and 100% ethanol, 5x2', xylene 2 x 10'

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FAQs for MYH6 Antibody (3-48) - Azide and BSA Free

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  • Q:

    We are looking for a Myosin heavy chain antibody. The sample is from mouse, and I would like to perform ICC. I found NB300-284. From its specific reference (https://www.sciencedirect.com/science/article/abs/pii/S0171933511002123), there are ICC images. However, I cannot confirm if those ICC images are produced by NB300-284. Is it possible for you to confirm for us?

    A: The catalog # NB300-284 refers to our myosin heavy chain antibody (3-48) and this antibody is generated from clone 3-48. If you see page number 151 of the publication (Eur J Cell Biol. 2012 Feb;91(2):150-5. Epub 2011 Dec 10), you can see a mention on Novus's myosin heavy chain antibody with clone 3-48 that was used for ICC-IF and WB applications.

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