MYL7 Antibody (OTI4E7) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-72859

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4E7

Format

Azide and BSA Free
View all MYL7 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human MYL7 (NP_067046) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

19.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MYL7 Antibody (OTI4E7) - Azide and BSA Free

Western Blot: MYL7 Antibody (OTI4E7)Azide and BSA Free [NBP2-72859]

Western Blot: MYL7 Antibody (OTI4E7)Azide and BSA Free [NBP2-72859]

Western Blot: MYL7 Antibody (OTI4E7) - Azide and BSA Free [NBP2-72859] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MYL7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MYL7.
Immunohistochemistry: MYL7 Antibody (OTI4E7) - Azide and BSA Free [NBP2-72859]

Immunohistochemistry: MYL7 Antibody (OTI4E7) - Azide and BSA Free [NBP2-72859]

Immunohistochemistry: MYL7 Antibody (OTI4E7) - Azide and BSA Free [NBP2-72859] - Staining of paraffin-embedded Human Kidney tissue using anti-MYL7 mouse monoclonal antibody.
Flow Cytometry: MYL7 Antibody (OTI4E7) - Azide and BSA Free [NBP2-72859]

Flow Cytometry: MYL7 Antibody (OTI4E7) - Azide and BSA Free [NBP2-72859]

Flow Cytometry: MYL7 Antibody (OTI4E7) - Azide and BSA Free [NBP2-72859] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-MYL7 antibody, and then analyzed by flow cytometry.
Immunohistochemistry: MYL7 Antibody (OTI4E7) - Azide and BSA Free [NBP2-72859]

Immunohistochemistry: MYL7 Antibody (OTI4E7) - Azide and BSA Free [NBP2-72859]

Immunohistochemistry: MYL7 Antibody (OTI4E7) - Azide and BSA Free [NBP2-72859] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MYL7 mouse monoclonal antibody.
Immunohistochemistry: MYL7 Antibody (OTI4E7) - Azide and BSA Free [NBP2-72859]

Immunohistochemistry: MYL7 Antibody (OTI4E7) - Azide and BSA Free [NBP2-72859]

Immunohistochemistry: MYL7 Antibody (OTI4E7) - Azide and BSA Free [NBP2-72859] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MYL7 mouse monoclonal antibody.
Immunohistochemistry: MYL7 Antibody (OTI4E7) - Azide and BSA Free [NBP2-72859]

Immunohistochemistry: MYL7 Antibody (OTI4E7) - Azide and BSA Free [NBP2-72859]

Immunohistochemistry: MYL7 Antibody (OTI4E7) - Azide and BSA Free [NBP2-72859] - Staining of paraffin-embedded Human liver tissue using anti-MYL7 mouse monoclonal antibody.

Applications for MYL7 Antibody (OTI4E7) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Western Blot

1:2000

Flow Cytometry Panel Builder

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MYL7

Myosins are a large family of motor proteins that share the common features of ATP hydrolysis, actin binding andpotential for kinetic energy transduction. Originally isolated from muscle cells (hence the name), almost alleukaryotic cells are now known to contain myosins. Structurally, mysoins contain a head domain that binds to actinfilaments (microfilaments) and is the site of ATP hydrolysis. The tail domain interacts with cargo molecules, and theneck acts as a linker between the head and tail and is the site of regulatory myosin light chain binding. There are 17myosin families and the most well characterized is myosin II. Myosin II is found predominantly in myocytes andmediates plus-ended movement along microfilaments. It is involved in muscle contraction through cyclic interactionswith actin-rich thin filaments, creating a contractile force. It is regulated by phosphorylation via myosin lightchain kinase (MLCK) and by intracellular Ca2+ concentrations.

Alternate Names

atrial isoform, light polypeptide 7, regulatory, MLC-2a, MYL2A, MYLC2AMLC2a, myosin, light chain 7, regulatory

Gene Symbol

MYL7

Additional MYL7 Products

Product Documents for MYL7 Antibody (OTI4E7) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for MYL7 Antibody (OTI4E7) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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