Myocilin Antibody (4F8) - Azide and BSA Free

Novus Biologicals | Catalog # H00004653-M01

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunoprecipitation, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4F8

Format

Azide and BSA Free
View all Myocilin Primary Antibodies »

Product Specifications

Immunogen

MYOC (AAH29261, 36 a.a. ~ 135 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. QLRKANDQSGRCQYTFSVASPNESSCPEQSQAMSVIHNLQRDSSTQRLDLEATKARLSSLESLLHQLTLDQAARPQETQEGLQRELGTLRRERDQLETQT

Specificity

MYOC - myocilin, trabecular meshwork inducible glucocorticoid response

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Myocilin Antibody (4F8) - Azide and BSA Free

Western Blot: Myocilin Antibody (4F8) [H00004653-M01]

Western Blot: Myocilin Antibody (4F8) [H00004653-M01]

Western Blot: Myocilin Antibody (4F8) [H00004653-M01] - Analysis of MYOC expression in transfected 293T cell line by MYOC monoclonal antibody (M01), clone 4F8.Lane 1: MYOC transfected lysate(57 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: Myocilin Antibody (4F8) [H00004653-M01]

Immunohistochemistry-Paraffin: Myocilin Antibody (4F8) [H00004653-M01]

Immunohistochemistry-Paraffin: Myocilin Antibody (4F8) [H00004653-M01] - Analysis of monoclonal antibody to MYOC on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.
Western Blot: Myocilin Antibody (4F8) [H00004653-M01]

Western Blot: Myocilin Antibody (4F8) [H00004653-M01]

Western Blot: Myocilin Antibody (4F8) [H00004653-M01] - Analysis of MYOC over-expressed 293 cell line, cotransfected with MYOC Validated Chimera RNAi ( Cat # H00004653-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MYOC monoclonal antibody (M01), clone 4F8 (Cat # H00004653-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Immunoprecipitation: Myocilin Antibody (4F8) [H00004653-M01]

Immunoprecipitation: Myocilin Antibody (4F8) [H00004653-M01]

Immunoprecipitation: Myocilin Antibody (4F8) [H00004653-M01] - Analysis of MYOC transfected lysate using anti-MYOC monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MYOC MaxPab rabbit polyclonal antibody.
ELISA: Myocilin Antibody (4F8) [H00004653-M01]

ELISA: Myocilin Antibody (4F8) [H00004653-M01]

ELISA: Myocilin Antibody (4F8) [H00004653-M01] - Detection limit for recombinant GST tagged MYOC is approximately 0.3ng/ml as a capture antibody.

Applications for Myocilin Antibody (4F8) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for IHC-P, RNAi Validation and ELISA.

Reviewed Applications

Read 1 review rated 5 using H00004653-M01 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Myocilin

MYOC encodes the protein myocilin, which is believed to have a role in cytoskeletal function. MYOC is expressed in many occular tissues, including the trabecular meshwork, and was revealed to be the trabecular meshwork glucocorticoid-inducible response protein (TIGR). The trabecular meshwork is a specialized eye tissue essential in regulating intraocular pressure, and mutations in MYOC have been identified as the cause of hereditary juvenile-onset open-angle glaucoma.

Alternate Names

GLC1A, MYOC, TIGR

Entrez Gene IDs

4653 (Human)

Gene Symbol

MYOC

UniProt

AAH29261 (Human)

Additional Myocilin Products

Product Documents for Myocilin Antibody (4F8) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Myocilin Antibody (4F8) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Wnt Signaling Modulators

Citations for Myocilin Antibody (4F8) - Azide and BSA Free

Customer Reviews for Myocilin Antibody (4F8) - Azide and BSA Free (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Myocilin Antibody (4F8) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Myocilin Antibody (4F8)
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Pig trabecular meshwork cells
    Species: Pig
    Verified Customer | Posted 12/05/2016
    Detection of Myocilin in cultured pig trabecular meshwork cells using Myocilin Antibody (4F8) (H00004653-M01) at 1:500 dilution overnight and donkey anti-mouse Alexa 488 (1:1000) for 1 hour.
    Myocilin Antibody (4F8) - Azide and BSA Free H00004653-M01

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies