Myoglobin Antibody (4F8) - Azide and BSA Free

Novus Biologicals | Catalog # H00004151-M13

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4F8

Format

Azide and BSA Free
View all Myoglobin Primary Antibodies »

Product Specifications

Immunogen

MB (NP_005359.1, 2 a.a. ~ 100 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GLSDGEWQLVLNVWGKVEADIPGHGQEVLIRLFKGHPETLEKFDKFKHLKSEDEMKASEDLKKHGATVLTALGGILKKKGHHEAEIKPLAQSHATKHKI

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Myoglobin Antibody (4F8) - Azide and BSA Free

Western Blot: Myoglobin Antibody (4F8) [H00004151-M13]

Western Blot: Myoglobin Antibody (4F8) [H00004151-M13]

Western Blot: Myoglobin Antibody (4F8) [H00004151-M13] - Analysis of MB expression in transfected 293T cell line by MB monoclonal antibody (M13), clone 4F8. Lane 1: MB transfected lysatE (17.2 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: Myoglobin Antibody (4F8) [H00004151-M13]

Immunocytochemistry/ Immunofluorescence: Myoglobin Antibody (4F8) [H00004151-M13]

Immunocytochemistry/Immunofluorescence: Myoglobin Antibody (4F8) [H00004151-M13] - Analysis of monoclonal antibody to MB on HeLa cell. Antibody concentration 10 ug/ml
Sandwich ELISA: Myoglobin Antibody (4F8) [H00004151-M13] - Detection limit for recombinant GST tagged MB is 0.03 ng/ml as a capture antibody.

Applications for Myoglobin Antibody (4F8) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
It has been used for ELISA and WB.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Myoglobin

Myoglobin is a small heme containing protein (153 amino acid residues, molecular weight (w/o heme) 17053 Da and theoretical pI=7.29) responsible for the oxygen deposition in muscle tissues. Only one form of myoglobin is expressed in cardiac and skeletal muscles. Myoglobin is known as a marker of myocardial damage and it has been used for more than three decades. Nowadays it still is very commonly used in clinical practice as an early marker of AMI. It appears in patient's blood 1 to 3 hours after onset of the symptoms, reaching peak level within 8 to 12 hours. Myoglobin is not so cardiac specific as cTnI or cTnT. Because of high myoglobin concentration in skeletal muscle tissue, even minor skeletal muscle injury results in the significant increase of myoglobin concentration in blood. Thus myoglobin is used together with cTnI or cTnT in clinical practise for better specificity in AMI diagnosis.

Alternate Names

MB

Gene Symbol

MB

UniProt

NP_005359.1 (Human)

Additional Myoglobin Products

Product Documents for Myoglobin Antibody (4F8) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Myoglobin Antibody (4F8) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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