Myosin light chain kinase Antibody (SU40-06)

Novus Biologicals | Catalog # NBP2-67486

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SU40-06 expressed in HEK293
View all Myosin light chain kinase Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human Myosin light chain kinase aa 1,865-1,914 / 1,914. (SwissProt: Q15746 Human; SwissProt: Q6PDN3 Mouse; SwissProt: D3ZFU9 Rat)

Localization

Cytoplasm, Cell projection, Cleavage furrow, Cytoskeleton.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Myosin light chain kinase Antibody (SU40-06)

Western Blot: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486]

Western Blot: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486]

Western Blot: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486] - Western blot analysis of Myosin light chain kinase on human lung tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperatur
Immunocytochemistry/ Immunofluorescence: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486]

Immunocytochemistry/ Immunofluorescence: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486]

Immunocytochemistry/Immunofluorescence: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486] - Staining MYLK in L6 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486]

Immunohistochemistry-Paraffin: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486]

Immunohistochemistry-Paraffin: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486] - Analysis of paraffin-embedded rat smooth muscle tissue using anti-MYLK antibody. Counter stained with hematoxylin.
Flow Cytometry: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486]

Flow Cytometry: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486]

Flow Cytometry: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486] - Analysis of SH-SY-5Y cells with MYLK antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western Blot: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486]

Western Blot: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486]

Western Blot: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486] - Analysis of MYLK on human lung lysates using anti-MYLK antibody at 1/5,000 dilution.
Immunocytochemistry/ Immunofluorescence: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486]

Immunocytochemistry/ Immunofluorescence: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486]

Immunocytochemistry/Immunofluorescence: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486] - Staining MYLK in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/ Immunofluorescence: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486]

Immunocytochemistry/ Immunofluorescence: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486]

Immunocytochemistry/Immunofluorescence: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486] - Staining MYLK in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/ Immunofluorescence: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486]

Immunocytochemistry/ Immunofluorescence: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486]

Immunocytochemistry/Immunofluorescence: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486] - Staining MYLK in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486]

Immunohistochemistry-Paraffin: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486]

Immunohistochemistry-Paraffin: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486] - Analysis of paraffin-embedded human colon cancer tissue using anti-MYLK antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486]

Immunohistochemistry-Paraffin: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486]

Immunohistochemistry-Paraffin: Myosin light chain kinase Antibody (SU40-06) [NBP2-67486] - Analysis of paraffin-embedded mouse smooth muscle tissue using anti-MYLK antibody. Counter stained with hematoxylin.

Applications for Myosin light chain kinase Antibody (SU40-06)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:800

Western Blot

1:1000-1:10000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Myosin light chain kinase

Myosin light chain kinase (MLCK) is a serine/threonine kinase that belongs to CaM Kinases (Ca++/Calmodulin-dependent protein kinases) family. MLCK is involved in the regulation of myosin activity during smooth muscle contraction. At increased level of Ca2+, rapid binding of Ca2+ to Calmodulin (CaM) occurs, triggering MLCK activation. Once activated, MLCK phosphorylates myosin light chain (MLC) and leading to cross-bridges cycling and generation of the contractile force (1). MLCK has been implicated in the regulation of endothelial and vascular permeability and in signaling pathway which leads to fibroblast apoptosis (2).

Long Name

Myosin light chain kinase, smooth muscle

Alternate Names

KRP, MLCK1, MYLK, MYLK1, smMLCK

Gene Symbol

MYLK

Additional Myosin light chain kinase Products

Product Documents for Myosin light chain kinase Antibody (SU40-06)

Certificate of Analysis

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Product Specific Notices for Myosin light chain kinase Antibody (SU40-06)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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