Myosin light chain kinase Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87744

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (92%), Rat (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Myosin light chain kinase Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KCVAKNDAGQAECSCQVTVDDAPASENTKAPEMKSRRPKSSLPPVLGTESDATVKKKPAPKTPPKAAMPPQIIQFPEDQKVRAGESVELFGKVTGTQPITCTWMKFRKQIQESEHMKVENSENGSKLTILAARQEHCGCYTLLVE

Marker

Slow Myosin, Heavy Chain Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Myosin light chain kinase Antibody - BSA Free

Immunohistochemistry-Paraffin: Myosin light chain kinase Antibody [NBP1-87744]

Immunohistochemistry-Paraffin: Myosin light chain kinase Antibody [NBP1-87744]

Immunohistochemistry-Paraffin: Myosin light chain kinase Antibody [NBP1-87744] - Staining in human smooth muscle and skeletal muscle tissues using anti-MYLK antibody. Corresponding MYLK RNA-seq data are presented for the same tissues.
Western Blot: Myosin light chain kinase Antibody [NBP1-87744]

Western Blot: Myosin light chain kinase Antibody [NBP1-87744]

Western Blot: Myosin light chain kinase Antibody [NBP1-87744] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: Human cell line HEL
Immunohistochemistry-Paraffin: Myosin light chain kinase Antibody [NBP1-87744]

Immunohistochemistry-Paraffin: Myosin light chain kinase Antibody [NBP1-87744]

Immunohistochemistry-Paraffin: Myosin light chain kinase Antibody [NBP1-87744] - Staining of human skeletal muscle shows low expression as expected.
Immunohistochemistry-Paraffin: Myosin light chain kinase Antibody [NBP1-87744]

Immunohistochemistry-Paraffin: Myosin light chain kinase Antibody [NBP1-87744]

Immunohistochemistry-Paraffin: Myosin light chain kinase Antibody [NBP1-87744] - Staining of human smooth muscle shows high expression.

Applications for Myosin light chain kinase Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Myosin light chain kinase

Myosin light chain kinase (MLCK) is a serine/threonine kinase that belongs to CaM Kinases (Ca++/Calmodulin-dependent protein kinases) family. MLCK is involved in the regulation of myosin activity during smooth muscle contraction. At increased level of Ca2+, rapid binding of Ca2+ to Calmodulin (CaM) occurs, triggering MLCK activation. Once activated, MLCK phosphorylates myosin light chain (MLC) and leading to cross-bridges cycling and generation of the contractile force (1). MLCK has been implicated in the regulation of endothelial and vascular permeability and in signaling pathway which leads to fibroblast apoptosis (2).

Long Name

Myosin light chain kinase, smooth muscle

Alternate Names

KRP, MLCK1, MYLK, MYLK1, smMLCK

Gene Symbol

MYLK

Additional Myosin light chain kinase Products

Product Documents for Myosin light chain kinase Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Myosin light chain kinase Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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