MYST1 Antibody (6U5Z6)

Novus Biologicals | Catalog # NBP3-16178

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6U5Z6 expressed in HEK293
View all MYST1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 350-458 of human MYST1 (Q9H7Z6). EKPLSDLGKLSYRSYWSWVLLEILRDFRGTLSIKDLSQMTSITQNDIISTLQSLNMVKYWKGQHVICVTPKLVEEHLKSAQYKKPPITVDSVCLKWAPPKHKQVKLSKK

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MYST1 Antibody (6U5Z6)

Western Blot: MYST1 Antibody (6U5Z6) [NBP3-16178]

Western Blot: MYST1 Antibody (6U5Z6) [NBP3-16178]

Western Blot: MYST1 Antibody (6U5Z6) [NBP3-16178] - Western blot analysis of extracts of various cell lines, using MYST1 Rabbit mAb (NBP3-16178) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.
Immunohistochemistry-Paraffin: MYST1 Antibody (6U5Z6) [NBP3-16178]

Immunohistochemistry-Paraffin: MYST1 Antibody (6U5Z6) [NBP3-16178]

Immunohistochemistry-Paraffin: MYST1 Antibody (6U5Z6) [NBP3-16178] - Immunohistochemistry of paraffin-embedded human appendix using MYST1 Rabbit mAb (NBP3-16178) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
MYST1 Antibody (6U5Z6)

Immunocytochemistry/ Immunofluorescence: MYST1 Antibody (6U5Z6) [NBP3-16178] -

Immunocytochemistry/ Immunofluorescence: MYST1 Antibody (6U5Z6) [NBP3-16178] - Confocal imaging of U-2 OS cells using MYST1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L).The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.
MYST1 Antibody (6U5Z6)

Immunocytochemistry/ Immunofluorescence: MYST1 Antibody (6U5Z6) [NBP3-16178] -

Immunocytochemistry/ Immunofluorescence: MYST1 Antibody (6U5Z6) [NBP3-16178] - Confocal imaging of paraffin-embedded Rat kidney using MYST1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Applications for MYST1 Antibody (6U5Z6)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MYST1

The MYST family of histone acetyltransferases, which includes MYST1, is named for the founding members MOZ (MYST3; MIM 601408), yeast YBF2 and SAS2, and TIP60 (HTATIP; MIM 601409). All members of this family contain a MYST region of about 240 amino acids with a canonical acetyl-CoA-binding site and a C2HC-type zinc finger motif. Most MYST proteins also have a chromodomain involved in protein-protein interactions and targeting transcriptional regulators to chromatin (Neal et al., 2000).

Alternate Names

FLJ14040, hMOFhistone acetyltransferase MYST1, KAT8, MOFEC 2.3.1.48, MOZ, YBF2/SAS3, SAS2 and TIP60 protein 1, MYST histone acetyltransferase 1, MYST-1, ortholog of Drosophila males absent on the first (MOF), probable histone acetyltransferase MYST1

Gene Symbol

KAT8

Additional MYST1 Products

Product Documents for MYST1 Antibody (6U5Z6)

Certificate of Analysis

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Product Specific Notices for MYST1 Antibody (6U5Z6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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