N-Acetyl-D-Glucosamine Kinase/NAGK Antibody (4D12) - BSA Free

Novus Biologicals | Catalog # NBP2-22554

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4D12

Format

BSA Free
View all N-Acetyl-D-Glucosamine Kinase/NAGK Primary Antibodies »

Product Specifications

Immunogen

Recombinant human N-Acetyl-D-Glucosamine Kinase/NAGK (1-344aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for N-Acetyl-D-Glucosamine Kinase/NAGK Antibody (4D12) - BSA Free

Western Blot: N-Acetyl-D-Glucosamine Kinase/NAGK Antibody (4D12)BSA Free [NBP2-22554]

Western Blot: N-Acetyl-D-Glucosamine Kinase/NAGK Antibody (4D12)BSA Free [NBP2-22554]

Western Blot: N-Acetyl-D-Glucosamine Kinase/NAGK Antibody (4D12) [NBP2-22554] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NAGK antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Immunocytochemistry/ Immunofluorescence: N-Acetyl-D-Glucosamine Kinase/NAGK Antibody (4D12) - BSA Free [NBP2-22554]

Immunocytochemistry/ Immunofluorescence: N-Acetyl-D-Glucosamine Kinase/NAGK Antibody (4D12) - BSA Free [NBP2-22554]

Immunocytochemistry/Immunofluorescence: N-Acetyl-D-Glucosamine Kinase/NAGK Antibody (4D12) [NBP2-22554] - Analysis of NAGK in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human NAGK antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Flow Cytometry: N-Acetyl-D-Glucosamine Kinase/NAGK Antibody (4D12) - BSA Free [NBP2-22554]

Flow Cytometry: N-Acetyl-D-Glucosamine Kinase/NAGK Antibody (4D12) - BSA Free [NBP2-22554]

Flow Cytometry: N-Acetyl-D-Glucosamine Kinase/NAGK Antibody (4D12) [NBP2-22554] - Analysis of NAGK in HeLa cell line, staining at 2-5ug for 1x106cells. The secondary antibody used goat antimouseIgG Alexa fluor 488 conjugate.

Applications for N-Acetyl-D-Glucosamine Kinase/NAGK Antibody (4D12) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: N-Acetyl-D-Glucosamine Kinase/NAGK

NAGK is a member of the eukaryotic-type N-acetylglucosamine kinase family. NAGK catalyzes the first committed step in arginine biosynthesis in organisms that perform the cyclic pathway of ornithine synthesis. In eukaryotic and bacterial oxygenic phototrophs, the activity of NAGK is controlled by the P(II) signal transduction protein. NAGK converts endogenous N-acetylglucosamine (GlcNAc), a key component of complex carbohydrates, from lysosomal degradation or alimentary sources into GlcNAc 6-phosphate.

Long Name

N-Acetyl-D-Glucosamine Kinase

Alternate Names

GlcNAc Kinase, GNK, N-Acetylglucosamine Kinase

Gene Symbol

NAGK

UniProt

NP_060037.2 (Human)

Additional N-Acetyl-D-Glucosamine Kinase/NAGK Products

Product Documents for N-Acetyl-D-Glucosamine Kinase/NAGK Antibody (4D12) - BSA Free

Certificate of Analysis

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Product Specific Notices for N-Acetyl-D-Glucosamine Kinase/NAGK Antibody (4D12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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