N-WASP Antibody - BSA Free

Western Blot: N-WASP Antibody [NBP1-82512]

Western Blot: N-WASP Antibody [NBP1-82512] - Analysis in human cell line RT-4.

Immunohistochemistry-Paraffin: N-WASP Antibody [NBP1-82512]

Immunohistochemistry-Paraffin: N-WASP Antibody [NBP1-82512] - Staining of human cerebral cortex shows moderate to strong positivity in neuronal cells.

Immunohistochemistry-Paraffin: N-WASP Antibody [NBP1-82512]

Immunohistochemistry-Paraffin: N-WASP Antibody [NBP1-82512] - Staining of human kidney shows moderate to strong positivity in cells in tubules and glomeruli.

Immunohistochemistry-Paraffin: N-WASP Antibody [NBP1-82512]

Immunohistochemistry-Paraffin: N-WASP Antibody [NBP1-82512] - Staining of human skeletal muscle shows weak positivity in myocytes.

Immunohistochemistry-Paraffin: N-WASP Antibody [NBP1-82512]

Immunohistochemistry-Paraffin: N-WASP Antibody [NBP1-82512] - Staining of human stomach shows moderate to strong positivity in glandular cells.

Simple Western: N-WASP Antibody [NBP1-82512]

Simple Western: N-WASP Antibody [NBP1-82512] - Simple Western lane view shows a specific band for N-WASP in 0.2 mg/ml of RT-4 (Left) and U-251MG (Right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Western Blot: N-WASP Antibody [NBP1-82512] -

Western Blot: N-WASP Antibody [NBP1-82512] - Generation of nWASP knockdown cell lines. A-549 & SK-MES-1 cells were treated with nWASP siRNA/non-targeting siRNA (NT) at 0.5 μg/ml & then analysed for nWASP expression after 48 h. a QPCR analysis of nWASP transcript expression demonstrates a significant decrease in expression in siRNA treated cells, n = 4 replicates. b PCR also demonstrates a decrease in nWASP expression. c Quantitative analysis of Western blots (n = 4) shows significant decrease in nWASP protein expression in both A-549 & SK-MES1 cell lines after 48 h siRNA treatment. d Representative image showing knockdown of nWASP at protein level in siRNA treated cells at 48 h Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28351346), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: N-WASP Antibody [NBP1-82512] -

Western Blot: N-WASP Antibody [NBP1-82512] - nWASP activity affects A-549 & SK-MES-1 cell growth. a, b nWASP inhibition using 10 μM wiskostatin treatment significantly impairs the growth of A-549 & SK-MES-1 cells, respectively. c, d A significant effect on growth is also evident after 3 days in nWASP knockdown A-549 & SK-MES-1 cells, respectively, when compared to non-targeting control treated cells Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28351346), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: N-WASP Antibody - BSA Free [NBP1-82512] -

N-WASP and TOCA-1 play a role in C.t. pedestal-like structure formation. (A) N-WASP and TOCA-1 were knocked down in HeLa cells. Knockdown was verified using Western blotting, probing with anti-GAPDH, anti-N-WASP, and anti-TOCA-1 antibodies and quantified using densitometry, with relative density or adjusted relative density shown under the blots. Relative density was determined compared to mock KD, and relative density was adjusted for the N-WASP and TOCA-1 blots compared to the GAPDH standards. (B) HeLa cells were asynchronously infected with WTL2 at an MOI of 50 for 15 minutes and imaged with transmission electron microscopy; three representative images are shown. (C) Quantification of EBs associated with pedestal-like structures. A total of 100 EBs per experiment were assessed from two separate experiments, in which images were blinded and categorized as associated or not associated with pedestal-like structures. EBs associated with pedestal-like structures were compared to total EBs to determine the percentage associated with pedestal-like structures. Data represent the mean of two biological replicates. Error bars represent SD, *P < 0.05. Significance was determined using one-way ANOVA followed by Tukey’s multiple comparisons test. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40231845), licensed under a CC-BY license. Not internally tested by Novus Biologicals.