N-WASP [p Ser484, p Ser485] Antibody

Novus Biologicals | Catalog # NBP2-29669

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Rat

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all N-WASP Primary Antibodies »

Product Specifications

Immunogen

Phospho-N-WASP (S484/S485) synthetic peptide (coupled to KLH) corresponding to amino acid residues around serine 484 and 485 of human N-WASP. The human WASP sequence has a similar peptide sequence surrounding serine 483 and 484.

Epitope

Phosphoserine 484 / Phosphserine 485

Modification

p Ser484, p Ser485

Specificity

The antibody detects a 63 kDa protein corresponding to the molecular weight of phosphorylated WASP on SDS-PAGE immunoBlots of Jurkat cell lysate. In rat brain, A431, human endothelial and SKN-SH cells, this antibody detects a 65 kDa protein corresponding to phosphorylated N-WASP.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Relevant Entrez Gene Numbers: NM_003941.2

Scientific Data Images for N-WASP [p Ser484, p Ser485] Antibody

Western Blot: N-WASP [p Ser484, p Ser485] Antibody [NBP2-29669]

Western Blot: N-WASP [p Ser484, p Ser485] Antibody [NBP2-29669]

Western Blot: N-WASP [p Ser484, p Ser485] Antibody [NBP2-29669] - Western blot of control and alkaline phosphatase-treated (AP) neonatal rat brain lysate (20 ug/lane). Blots were probed with anti-N-WASP (Lanes 1 and 2), anti-phospho-N-WASP (S484/S485) (Lanes 3 and 4), or anti-unphosphorylated-N-WASP (S484/S485) (Lanes 5 and 6).

Applications for N-WASP [p Ser484, p Ser485] Antibody

Application
Recommended Usage

ELISA

1:2000

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

100 ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA.

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: N-WASP

Members of the Wiskott-Aldrich sydrome protein (WASP) family regulate the formation of actin-based cell structures in many cell types. These proteins contain C-terminal actin-binding domains that can stimulate actin polymerization. In addition, these proteins bind the ARP2/3 complex, which can nucleate actin polymerization at sites that lead to branched actin structures. WASP is expressed primarily in hematopoietic cells, while its homolog N-WASP is widely expressed. These proteins have 48% identity in human with the highest homology in the functional regions of these proteins. Phosphorylation regulates the activity of both proteins. Dual phosphorylation of WASP on serine 483 and 484 by casein kinases increase the affinity for the ARP2/3 complex. Thus, dual serine phosphorylation may be important for formation of actin-based structures in various cell types.

Long Name

Neural Wiskott-Aldrich Syndrome Protein

Alternate Names

NWASP, TRS4, WASL

Gene Symbol

WASL

UniProt

O00401

Additional N-WASP Products

Product Documents for N-WASP [p Ser484, p Ser485] Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for N-WASP [p Ser484, p Ser485] Antibody

Relevant Entrez Gene Numbers: NM_003941.2

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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