N-WASP [p Ser485, p Ser484] Antibody

Name: N-WASP [p Ser485, p Ser484] Antibody
Brand: Novus Biologicals
SKU: NBP3-23284
Price: 399 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-23284

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all N-WASP Primary Antibodies »

Product Specifications

Immunogen

Phospho-N-WASP (S484/S485) synthetic peptide (coupled to KLH) corresponding to amino acid residues around serine 484 and 485 of human N-WASP. The human WASP sequence has a similar peptide sequence surrounding serine 483 and 484. [UniProt# O00401]

Modification

p Ser485, p Ser484

Specificity

This antibody was cross-adsorbed to unphosphorylated-N-WASP (S484/S485) peptide then affinity purified using phospho-N-WASP (S484/S485) peptide. The antibody detects a 63 kDa protein corresponding to the molecular mass of phosphorylated WASP on SDS-PAGE immunoblots of Jurkat cell lysate. In rat brain, A431, human endothelial and SKN-SH cells, this antibody detects a 65 kDa protein corresponding to phosphorylated N-WASP.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

63 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for N-WASP [p Ser485, p Ser484] Antibody

Immunocytochemistry/Immunofluorescence: N-WASP [p Ser485, p Ser484] Antibody [NBP3-23284] -

Immunocytochemistry/Immunofluorescence: N-WASP [p Ser485, p Ser484] Antibody [NBP3-23284] - Immunocytochemical labeling of phosphorylated and unphosphorylated N-WASP in rabbit spleen fibroblasts. The cells were probed with N-WASP (Ser-484/Ser-485) phospho-specific and N-WASP (Ser-484/Ser-485) unphosphorylated antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3. The antibodies were used in the absence (left) or presence (right) of their respective blocking peptide.

Western Blot: N-WASP [p Ser485, p Ser484] Antibody [NBP3-23284] -

Western Blot: N-WASP [p Ser485, p Ser484] Antibody [NBP3-23284] - Western blot analysis of control and alkaline phosphatase-treated (AP) neonatal rat brain lysate (20 ug/lane). Blots were probed with anti-N-WASP (Lanes 1 & 2), anti-phospho-N-WASP (S484/S485) (Lanes 3 & 4), or anti-unphosphorylated-N-WASP (S484/S485) (Lanes 5 & 6).

Applications for N-WASP [p Ser485, p Ser484] Antibody

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: N-WASP

The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. The WASL gene product is a homolog of WAS protein, however, unlike the latter, it is ubiquitously expressed and shows highest expression in neural tissues. It has been shown to bind Cdc42 directly, and induce formation of long actin microspikes.

Long Name

Neural Wiskott-Aldrich Syndrome Protein

Alternate Names

NWASP, TRS4, WASL

Gene Symbol

WASL

Additional N-WASP Products

Product Documents for N-WASP [p Ser485, p Ser484] Antibody

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Product Specific Notices for N-WASP [p Ser485, p Ser484] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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