N6-methyladenosine (m6A) Antibody (6S5W2)

Name: N6-methyladenosine (m6A) Antibody (6S5W2)
Brand: Novus Biologicals
SKU: NBP3-35057
Price: 659 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-35057

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Non-species specific

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, RNA immunoprecipitation, Dot Blot

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 6S5W2

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Product Specifications

Immunogen

Chemical compounds corresponding to N6-methyladenosine (m6A).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for N6-methyladenosine (m6A) Antibody (6S5W2)

Immunocytochemistry/ Immunofluorescence: N6-methyladenosine (m6A) Antibody (6S5W2) [NBP3-35057] -

Immunocytochemistry/ Immunofluorescence: N6-methyladenosine (m6A) Antibody (6S5W2) [NBP3-35057] - Immunofluorescence analysis of U-2 OS treated with UV(30s)+Brdu(0min) and U-2 OS treated with UV(30s)+Brdu(10min) cells using N6-methyladenosine (m6A) Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Dot Blot: N6-methyladenosine (m6A) Antibody (6S5W2) [NBP3-35057] -

Dot Blot: N6-methyladenosine (m6A) Antibody (6S5W2) [NBP3-35057] - The m6A rabbit monoclonal antibody are tested in Dot Blot against N6-methyladenosine (m6A) and unmodified adenosine.
Oligomer 8 - ATAACTGG-m6A-CCGAATGG
Oligomer 9 - ATAACTGGACCGAATGG
Oligomer 10 - AAAAAAAAAAAAAAAA-biotin.

RNA immunoprecipitation: N6-methyladenosine (m6A) Antibody (6S5W2) [NBP3-35057] -

RNA immunoprecipitation: N6-methyladenosine (m6A) Antibody (6S5W2) [NBP3-35057] - RNA Immunoprecipitation was performed on 100 µg mouse liver total RNA,using 5 µg of the N6-methyladenosine / m6A Rabbit mAb. An equal amount of IgG was used as negative control. The immunoprecipitated RNA was verified by using HS6ST1 as PCR primer of qRT-PCR. The picture shows the relative enrichment multiple of HS6ST1 site.

Applications for N6-methyladenosine (m6A) Antibody (6S5W2)

Application

Recommended Usage

Dot Blot

1:500 - 1:2000

ELISA

Recommended starting concentration is 1 μg/mL

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

RNA immunoprecipitation

1:50 - 1:200

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: N6-methyladenosine (m6A)

Alternate Names

(-)-6-(Methylamino)purine riboside, N(6)-Methyladenosine, N-Methyladenosine

Additional N6-methyladenosine (m6A) Products

Product Documents for N6-methyladenosine (m6A) Antibody (6S5W2)

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Certificate of Analysis

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Product Specific Notices for N6-methyladenosine (m6A) Antibody (6S5W2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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