N6-methyladenosine (m6A) Antibody (7J1S3)
Novus Biologicals | Catalog # NBP3-33361
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Key Product Details
Species Reactivity
Non-species specific
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, RNA immunoprecipitation, Dot Blot
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 7J1S3
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Product Specifications
Immunogen
Chemical compounds corresponding to N6-methyladenosine (m6A).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for N6-methyladenosine (m6A) Antibody (7J1S3)
Immunocytochemistry/ Immunofluorescence: N6-methyladenosine (m6A) Antibody (7J1S3) [NBP3-33361] -
Immunocytochemistry/ Immunofluorescence: N6-methyladenosine (m6A) Antibody (7J1S3) [NBP3-33361] - U2OS cells pre-treated with BrdU were subjected UVC irradiation incubated at 37 C for the indicated time,immunofluorescence analysis was performed by N6-methyladenosine (m6A) Rabbit mAb,DAPI, 4',6-diamidino-2-phenylindole. Global UVC irradiation exceed cytoplasmic leavel,peaking at 2 min after irradiation and diminishing over the following 8 min.Dot Blot: N6-methyladenosine (m6A) Antibody (7J1S3) [NBP3-33361] -
Dot Blot: N6-methyladenosine (m6A) Antibody (7J1S3) [NBP3-33361] - Dot-blot analysis of all sorts of peptides using N6-methyladenosine (m6A) antibody at dilution.Dot Blot: N6-methyladenosine (m6A) Antibody (7J1S3) [NBP3-33361] -
Dot Blot: N6-methyladenosine (m6A) Antibody (7J1S3) [NBP3-33361] - The N6-methyladenosine / m6A Rabbit mAb are tested in Dot Blot against N6-methyladenosine (m6A) and unmodified adenosine.Oligomer 8 - ATAACTGG-m6A-CCGAATGG
Oligomer 9 - ATAACTGGACCGAATGG
Oligomer 10 - AAAAAAAAAAAAAAAA-biotin.
Dot Blot: N6-methyladenosine (m6A) Antibody (7J1S3) [NBP3-33361] -
Dot Blot: N6-methyladenosine (m6A) Antibody (7J1S3) [NBP3-33361] - The N6-methyladenosine (m6A) Rabbit mAb (4ug,A19841) are tested in Nucleotide Array against N6-methyladenosine (m6A) and unmodified adenosine (100pmol for each oligomer).Oligomer 4 - N6-methyladenosine (m6A-UAACUGGACCGAAUGG-Biotin)
Oligomer 5 - N6-methyladenosine
Oligomer 6 - unmodified adenosine
Dot Blot: N6-methyladenosine (m6A) Antibody (7J1S3) [N6-methyladenosine (m6A)] -
Dot Blot: N6-methyladenosine (m6A) Antibody (7J1S3) [N6-methyladenosine (m6A)] - The N6-methyladenosine (m6A) Rabbit mAb (4ug,A19841) are tested in Nucleotide Array against N6-methyladenosine (m6A) and unmodified adenosine (100pmol for each oligomer).Oligomer 4 - N6-methyladenosine (m6A-UAACUGGACCGAAUGG-Biotin)
Oligomer 5 - N6-methyladenosine
Oligomer 6 - unmodified adenosine
Applications for N6-methyladenosine (m6A) Antibody (7J1S3)
Application
Recommended Usage
Dot Blot
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 ug/mL
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
RNA immunoprecipitation
1:50 - 1:200
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: N6-methyladenosine (m6A)
Alternate Names
(-)-6-(Methylamino)purine riboside, N(6)-Methyladenosine, N-Methyladenosine
Additional N6-methyladenosine (m6A) Products
Product Documents for N6-methyladenosine (m6A) Antibody (7J1S3)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for N6-methyladenosine (m6A) Antibody (7J1S3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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