Nanog Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-24941

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Primate (96%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

A portion of amino acids 1-50 of human NANOG was used as the immunogen.

Marker

Embryonic Stem Cell Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Nanog Antibody - BSA Free

Western Blot: Nanog Antibody [NBP2-24941]

Western Blot: Nanog Antibody [NBP2-24941]

Western Blot: Nanog Antibody [NBP2-24941] - Analysis of Nanog in (A) HeLa and (B) NIH 3T3 cell lysate using NBP2-24941 at 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: Nanog Antibody [NBP2-24941]

Immunocytochemistry/ Immunofluorescence: Nanog Antibody [NBP2-24941]

Immunocytochemistry/Immunofluorescence: Nanog Antibody [NBP2-24941] - Nanog antibody was tested in Ntera2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). Image objective 40x. An antibody dilution of 1:10 was used.
Western Blot: Nanog Antibody [NBP2-24941]

Western Blot: Nanog Antibody [NBP2-24941]

Nanog-Antibody-Western-Blot-NBP2-24941-img0006.jpg
Nanog Antibody

Western Blot: Nanog Antibody [NBP2-24941] -

Protein expression of NANOG a, OCT4 b & SOX2 c in bovine uterine tissues obtained from control cows & from cows with adenomyosis. Data were normalized against glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Bars represent the mean ± SEM. There were no statistical differences between uterine normal & adenomyotic tissues (P > 0.05), as determined by Student’s t-test. Representative blots for NANOG, OCT4, SOX2 & GAPDH are shown below the graphs d. MM – molecular weight marker, C – tissues obtained from control cows, ADENO – tissues obtained from cows with adenomyosis Image collected & cropped by CiteAb from the following publication (http://www.rbej.com/content/13/1/110), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Nanog Antibody

Immunohistochemistry: Nanog Antibody [NBP2-24941] -

Immunohistochemistry: Nanog Antibody [NBP2-24941] - Immunodetection of pluripotency markers in uterine tissues from control cows & from cows with adenomyosis. a, b – haematoxylin & eosin stained slides of control & adenomyotic uterus, respectively; c, d – NANOG immunodetection in control & adenomyotic tissue, respectively; e, f – immunolocalisation of OCT4 in normal & adenomyotic tissue, respectively; g, h – SOX2 immunodetection in control & adenomyotic tissue, respectively; i, j, k – no Ab, negative controls for NANOG, OCT4 & SOX2, respectively. Unspecific IgG controls (pictures not shown) served similar pictures as no Ab control. Arrows indicate the most intense histochemical reactions; dotted line indicate endometrial-myometrial border; e – endometrium, m – myometrium, es – endometrial stroma, eg – endometrial gland, a – adenomyotic lesion, v – vessel. Scale bars: 20 μm Image collected & cropped by CiteAb from the following publication (http://www.rbej.com/content/13/1/110), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Nanog Antibody - BSA Free

Application
Recommended Usage

Flow Cytometry

reported in scientific literature (PMID 26283864)

Immunocytochemistry/ Immunofluorescence

1:10

Western Blot

0.5-2 ug/ml

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Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nanog

Nanog is one of the molecular markers suitable for recognizing the undifferentiated state of stem cells in the mouse and human. Nanog is a divergent homeodomain protein that directs propagation of undifferentiated ES cells. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Nanog-deficient ES cells lose pluripotency and differentiate into extraembryonic endoderm lineage.

Long Name

Nanog Homeobox

Alternate Names

FLJ12581, FLJ40451, hNanog, homeobox protein NANOG, Homeobox transcription factor Nanog, homeobox transcription factor Nanog-delta 48, Nanog homeobox

Gene Symbol

NANOG

UniProt

Additional Nanog Products

Product Documents for Nanog Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Nanog Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Nanog Antibody - BSA Free

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Protocols

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