NAT10 Antibody (5Z3E5)

Novus Biologicals | Catalog # NBP3-15742

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5Z3E5 expressed in HEK293
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Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 750-1025 of human NAT10 (Q9H0A0). KTLTDEDEADQGGWLAAFWKDFRRRFLALLSYQFSTFSPSLALNIIQNRNMGKPAQPALSREELEALFLPYDLKRLEMYSRNMVDYHLIMDMIPAISRIYFLNQLGDLALSAAQSALLLGIGLQHKSVDQLEKEIELPSGQLMGLFNRIIRKVVKLFNEVQEKAIEEQMVAAKDVVMEPTMKTLSDDLDEAAKEFQEKHKKEVGKLKSMDLSEYIIRGDDEEWNEVLNKAGPNASIISLKSDKKRKLEAKQEPKQSKKLKNRETKNKKDMKLKRKK

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NAT10 Antibody (5Z3E5)

Western Blot: NAT10 Antibody (5Z3E5) [NBP3-15742]

Western Blot: NAT10 Antibody (5Z3E5) [NBP3-15742]

Western Blot: NAT10 Antibody (5Z3E5) [NBP3-15742] - Analysis of extracts of Mouse testis, using NAT10 antibody (NBP3-15742) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
NAT10 Antibody (5Z3E5)

Immunohistochemistry: NAT10 Antibody (5Z3E5) [NAT10] -

Immunohistochemistry: NAT10 Antibody (5Z3E5) [NAT10] - Immunohistochemistry analysis of paraffin-embedded Human lung squamous carcinoma tissue using NAT10 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
NAT10 Antibody (5Z3E5)

Immunohistochemistry: NAT10 Antibody (5Z3E5) [NAT10] -

Immunohistochemistry: NAT10 Antibody (5Z3E5) [NAT10] - Immunohistochemistry analysis of paraffin-embedded Mouse liver using NAT10 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
NAT10 Antibody (5Z3E5)

Immunohistochemistry: NAT10 Antibody (5Z3E5) [NAT10] -

Immunohistochemistry: NAT10 Antibody (5Z3E5) [NAT10] - Immunohistochemistry analysis of paraffin-embedded Human colon using NAT10 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
NAT10 Antibody (5Z3E5)

Western Blot: NAT10 Antibody (5Z3E5) [NAT10] -

Western Blot: NAT10 Antibody (5Z3E5) [NAT10] - Western blot analysis of various lysates using NAT10 Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3s.
NAT10 Antibody (5Z3E5)

Immunohistochemistry: NAT10 Antibody (5Z3E5) [NAT10] -

Immunohistochemistry: NAT10 Antibody (5Z3E5) [NAT10] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using NAT10 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
NAT10 Antibody (5Z3E5)

Immunohistochemistry: NAT10 Antibody (5Z3E5) [NAT10] -

Immunohistochemistry: NAT10 Antibody (5Z3E5) [NAT10] - Immunohistochemistry analysis of paraffin-embedded Rat spleen using NAT10 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
NAT10 Antibody (5Z3E5)

Immunohistochemistry: NAT10 Antibody (5Z3E5) [NAT10] -

Immunohistochemistry: NAT10 Antibody (5Z3E5) [NAT10] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using NAT10 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Applications for NAT10 Antibody (5Z3E5)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NAT10

NAT10 has protein acetyltransferase activity in vitro. Can acetylate both histones and microtubules. Histone acetylation may regulate transcription and mitotic chromosome de-condensation. Activates telomerase activity by stimulating the transcription of TERT, a

Long Name

RNA cytidine acetyltransferase

Alternate Names

ALP, EC 2.3.1.-, hALP, KIAA1709

Gene Symbol

NAT10

Additional NAT10 Products

Product Documents for NAT10 Antibody (5Z3E5)

Certificate of Analysis

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Product Specific Notices for NAT10 Antibody (5Z3E5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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