NCAPH Antibody - BSA Free

Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88345]

Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88345] - Staining of human kidney shows no positivity as expected.

Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88345]

Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88345] - Staining of human kidney, skin, testis and tonsil using Anti-NCAPH antibody NBP1-88345 (A) shows similar protein distribution across tissues to independent antibody NBP1-88346 (B).

Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88345]

Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88345] - Staining of human skin shows nuclear positivity in epidermal cells.

Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88345]

Immunohistochemistry-Paraffin: NCAPH Antibody [NBP1-88345] - Staining of human tonsil shows nuclear positivity in germinal center cells.

Simple Western: NCAPH Antibody [NBP1-88345]

Simple Western: NCAPH Antibody [NBP1-88345] - Simple Western lane view shows a specific band for NCAPH in 0.2 mg/ml of RT-4 (Left) and U-251MG (Right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: NCAPH Antibody [NBP1-88345]

Simple Western: NCAPH Antibody [NBP1-88345] - Electropherogram image(s) of corresponding Simple Western lane view. NCAPH antibody was used at 1:20 dilution on RT-4 and U-251MG lysate(s).

Western Blot: NCAPH Antibody - BSA Free [NBP1-88345]

Analysis in human cell lines U2OS and SK-MEL-30 using Anti-NCAPH antibody. Corresponding NCAPH RNA-seq data are presented for the same cell lines. Loading control: Anti-COX4I1.

Western Blot: NCAPH Antibody - BSA Free [NBP1-88345]

Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-NCAPH antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Western Blot: NCAPH Antibody - BSA Free [NBP1-88345] -

MiR-1976 targets NCAPH 3’-UTR: 1627 bp-1633 bp. A549 and NCI-H1975 cells were transfected with corresponding mimics, respectively and real-time PCR was used to detect the NCAPH mRNA level (A). A549 and NCI-H1975 cells were transfected with corresponding inhibitors, respectively and real-time PCR was used to detect the NCAPH mRNA level (B). Western blot was used to analyze NCAPH protein levels in A549 and NCI-H1975 cells (C, D). A schematic diagram showed the three predicted potential binding sites of miR-1976 at the 3 ' -UTR of NCAPH (E). The luciferase reporter vector containing predicted three binding sites were cloned into pGL3-promoter, namely pGL3-T1, pGL3-T2, and pGL3-T3. The three vectors were co-transfected with miR-1976 mimic respectively into A549 cells, and luciferase signals were detected by luciferase assay (F). The pGL3-T2 was co-transfected with the miR-1976 mimic into A549 cells. Luciferase signals were detected by luciferase assay (G). The pGL3-T2 was co-transfected respectively with the miR-1976 inhibitor into A549 cells. Luciferase signals were detected by luciferase assay (H). The pGL3-T2 was co-transfected with the miR-1976 mimic into A549 cells. The pGL3-T2 mut was co-transfected with the miR-1976 mimic into A549 cells. Luciferase signal was detected by luciferase method (I). * P < 0.05, ** P < 0.01, ***P < 0.001. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41598-024-61261-6), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: NCAPH Antibody - BSA Free [NBP1-88345] -

NCAPH promotes proliferation &migration and inhibits apoptosis of LUAD cells. NCAPH overexpression/knockdown lentivirus was used to treat A549 and NCI-H1975 cells. NCAPH proteins were detected by western blot assay (A, C) and quantitative analysis (B, D). The viability of LUAD cells was detected by CCK8 assay (E–H). Transwell assays were performed to detect cell migration (I, J). Apoptosis was measured by flow cytometry (K, L). * P < 0.05, ** P < 0.01, ***P < 0.001. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41598-024-61261-6), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: NCAPH Antibody - BSA Free [NBP1-88345] -

MiR-1976 suppresses tumor growth & metastasis in vivo by targeting NCAPH. A549 cells were infected with the corresponding lentivirus, respectively. About 1 × 107 cells were implanted subcutaneously into nude mice (A), and the tumor volume (B) and weight(C) were observed after cell implantation. NCAPH protein level in xenograft tumor tissues was detected by Western blotting (D, E). In Fig. 7D, the experiments were repeated twice on one membrane (Supplementary information file 2), and Fig. 7D represented one experimental result. The expression of NCAPH, PCNA, and cleaved-caspase-3 in xenograft tumors was detected by IHC staining (F–I). PCNA is a marker of tumor proliferation. A549 cells infected with lentivirus were injected into the tail vein of nude mice (5 in each group) (J). The average number of tumor nodules in each group was calculated (K). HE staining was used to demonstrate tumor nodules (L). * P < 0.05, ** P < 0.01, ***P < 0.001. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41598-024-61261-6), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: NCAPH Antibody - BSA Free [NBP1-88345] -

NCAPH promotes proliferation &migration and inhibits apoptosis of LUAD cells. NCAPH overexpression/knockdown lentivirus was used to treat A549 and NCI-H1975 cells. NCAPH proteins were detected by western blot assay (A, C) and quantitative analysis (B, D). The viability of LUAD cells was detected by CCK8 assay (E–H). Transwell assays were performed to detect cell migration (I, J). Apoptosis was measured by flow cytometry (K, L). * P < 0.05, ** P < 0.01, ***P < 0.001. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41598-024-61261-6), licensed under a CC-BY license. Not internally tested by Novus Biologicals.