NCF4 Antibody (4L9Z4)

Novus Biologicals | Catalog # NBP3-15322

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4L9Z4 expressed in HEK293
View all NCF4 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 240-339 of human NCF4 (Q15080). LRCYYYEDTISTIKDIAVEEDLSSTPLLKDLLELTRREFQREDIALNYRDAEGDLVRLLSDEDVALMVRQARGLPSQKRLFPWKLHITQKDNYRVYNTMP

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NCF4 Antibody (4L9Z4)

Western Blot: NCF4 Antibody (4L9Z4) [NBP3-15322]

Western Blot: NCF4 Antibody (4L9Z4) [NBP3-15322]

Western Blot: NCF4 Antibody (4L9Z4) [NBP3-15322] - Western blot analysis of extracts of Mouse thymus, using NCF4 antibody (NBP3-15322) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
Immunohistochemistry-Paraffin: NCF4 Antibody (4L9Z4) [NBP3-15322]

Immunohistochemistry-Paraffin: NCF4 Antibody (4L9Z4) [NBP3-15322]

Immunohistochemistry-Paraffin: NCF4 Antibody (4L9Z4) [NBP3-15322] - Immunohistochemistry of paraffin-embedded human colon carcinoma using NCF4 Rabbit mAb (NBP3-15322) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Western Blot: NCF4 Antibody (4L9Z4) [NBP3-15322]

Western Blot: NCF4 Antibody (4L9Z4) [NBP3-15322]

Western Blot: NCF4 Antibody (4L9Z4) [NBP3-15322] - Western blot analysis of extracts of THP-1 cells, using NCF4 antibody (NBP3-15322) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunoprecipitation: NCF4 Antibody (4L9Z4) [NBP3-15322]

Immunoprecipitation: NCF4 Antibody (4L9Z4) [NBP3-15322]

Immunoprecipitation: NCF4 Antibody (4L9Z4) [NBP3-15322] - Immunoprecipitation analysis of 300ug extracts of THP-1 cells using 3ug NCF4 antibody (NBP3-15322). Western blot was performed from the immunoprecipitate using NCF4 antibody (NBP3-15322) at a dilition of 1:1000.

Applications for NCF4 Antibody (4L9Z4)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NCF4

The NADPH oxidase generates superoxide in phagocytic cells. It is important for immunity and its deficiency leads to chronic granulomatous disease (CGD) (1). p40-phox is a newly isolated cytosolic component of the nicotinamide adenine dinucleotide phosphate (NADPH)-oxidase that copurifies with p67-phox. Preliminary evidence indicates that it is a component of the cytosolic complex (2). Maximal activation of NADPH oxidase requires formation of a complex between the p40-phox and p67-phox subunits via association of their PB1 domains. The crystal structure of the p40-phox/p67phox PB1 heterodimer reveals that both domains have a beta grasp topology and that they bind in a front-to-back arrangement through conserved electrostatic interactions between an acidic OPCA motif on p40-phox and basic residues in p67-phox (3).

Alternate Names

MGC3810, NCF, NCF-4, neutrophil cytosol factor 4, neutrophil cytosolic factor 4 (40kD), neutrophil cytosolic factor 4, 40kDa, Neutrophil NADPH oxidase factor 4, p40phox, p40-phox, SH3 and PX domain-containing protein 4, SH3PXD4P40PHOX

Gene Symbol

NCF4

Additional NCF4 Products

Product Documents for NCF4 Antibody (4L9Z4)

Certificate of Analysis

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Product Specific Notices for NCF4 Antibody (4L9Z4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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