NCK1 Antibody (4R1N9)

Name: NCK1 Antibody (4R1N9)
Brand: Novus Biologicals
SKU: NBP3-16759
Price: 389 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-16759

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4R1N9 expressed in HEK293

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Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human NCK11 (P16333). PFSSSNDEELNFEKGDVMDVIEKPENDPEWWKCRKINGMVGLVPKNYVTVMQNNPLTSGLEPSPPQCDYIRPSLTGKFAGNPWYYGKVTRHQAEMALNERG

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NCK1 Antibody (4R1N9)

Western Blot: NCK1 Antibody (4R1N9) [NBP3-16759]

Western Blot: NCK1 Antibody (4R1N9) [NBP3-16759] - Western blot analysis of extracts of various cell lines, using NCK11 Rabbit mAb (NBP3-16759) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunocytochemistry/ Immunofluorescence: NCK1 Antibody (4R1N9) [NBP3-16759]

Immunocytochemistry/Immunofluorescence: NCK1 Antibody (4R1N9) [NBP3-16759] - Immunofluorescence analysis of NIH-3T3 cells using NCK11 Rabbit mAb (NBP3-16759) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunohistochemistry-Paraffin: NCK1 Antibody (4R1N9) [NBP3-16759]

Immunohistochemistry-Paraffin: NCK1 Antibody (4R1N9) [NBP3-16759] - Immunohistochemistry of paraffin-embedded rat testis using NCK11 Rabbit mAb (NBP3-16759) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence: NCK1 Antibody (4R1N9) [NBP3-16759] -

Immunocytochemistry/ Immunofluorescence: NCK1 Antibody (4R1N9) [NBP3-16759] - Immunofluorescence analysis of NIH-3T3 cells using NCK11 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Applications for NCK1 Antibody (4R1N9)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NCK1

Nck is a ubiquitously expressed adaptor protein consisting of one SH2 and three SH3 Src homology domains. Stimulation by epidermal growth factor (EGF) or platelet-derived growth factor (PDGF) leads to phosphorylation of Nck on tyrosine, serine and threonine residues. Overexpression of Nck resulted in transformation of NIH 3T3 and 3Y1 rat fibroblast (1-2). Additionally, Nck has been shown to associate with BLNK, PLCG1 and VAV1 in a B-cell antigen receptor-dependent fashion and may play a role in linking tyrosine and serine/threonine kinase pathways within the cell (2).

Long Name

Non-catalytic Region of Tyrosine Kinase Adaptor Protein 1

Alternate Names

cytoplasmic protein NCK1, MGC12668, NCK adaptor protein 1melanoma NCK protein, Nck-1, NCKalpha, NCKNCK tyrosine kinase, non-catalytic region of tyrosine kinase, SH2/SH3 adaptor protein NCK-alpha

Gene Symbol

NCK1

Additional NCK1 Products

Product Documents for NCK1 Antibody (4R1N9)

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Certificate of Analysis

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Product Specific Notices for NCK1 Antibody (4R1N9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Intracellular Kinases

PDGF Family

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