NCK1 Antibody (5B7) - BSA Free

Novus Biologicals | Catalog # NBP2-37490

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Monkey, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5B7

Format

BSA Free
View all NCK1 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human NCK1 expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NCK1 Antibody (5B7) - BSA Free

Western Blot: NCK1 Antibody (5B7)BSA Free [NBP2-37490]

Western Blot: NCK1 Antibody (5B7)BSA Free [NBP2-37490]

Western Blot: NCK1 Antibody (5B7) [NBP2-37490] - Western blot analysis using NCK1 mouse mAb against Jurkat (1), HeLa (2), HEK293 (3), A431 (4), K562 (5), and COS7 (6) cell lysate.
Immunohistochemistry: NCK1 Antibody (5B7) - BSA Free [NBP2-37490]

Immunohistochemistry: NCK1 Antibody (5B7) - BSA Free [NBP2-37490]

Immunohistochemistry: NCK1 Antibody (5B7) [NBP2-37490] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using NCK1 mouse mAb with DAB staining.
Flow Cytometry: NCK1 Antibody (5B7) - BSA Free [NBP2-37490]

Flow Cytometry: NCK1 Antibody (5B7) - BSA Free [NBP2-37490]

Flow Cytometry: NCK1 Antibody (5B7) [NBP2-37490] - Flow cytometric analysis of Jurkat cells using NCK1 mouse mAb (green) and negative control (red).
Western Blot: NCK1 Antibody (5B7)BSA Free [NBP2-37490]

Western Blot: NCK1 Antibody (5B7)BSA Free [NBP2-37490]

Western Blot: NCK1 Antibody (5B7) [NBP2-37490] - Western blot analysis using NCK1 mAb against human NCK1 (AA: 203-371) recombinant protein. (Expected MW is 44.9 kDa)
ELISA: NCK1 Antibody (5B7) - BSA Free [NBP2-37490]

ELISA: NCK1 Antibody (5B7) - BSA Free [NBP2-37490]

ELISA: NCK1 Antibody (5B7) [NBP2-37490] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for NCK1 Antibody (5B7) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NCK1

Nck is a ubiquitously expressed adaptor protein consisting of one SH2 and three SH3 Src homology domains. Stimulation by epidermal growth factor (EGF) or platelet-derived growth factor (PDGF) leads to phosphorylation of Nck on tyrosine, serine and threonine residues. Overexpression of Nck resulted in transformation of NIH 3T3 and 3Y1 rat fibroblast (1-2). Additionally, Nck has been shown to associate with BLNK, PLCG1 and VAV1 in a B-cell antigen receptor-dependent fashion and may play a role in linking tyrosine and serine/threonine kinase pathways within the cell (2).

Long Name

Non-catalytic Region of Tyrosine Kinase Adaptor Protein 1

Alternate Names

cytoplasmic protein NCK1, MGC12668, NCK adaptor protein 1melanoma NCK protein, Nck-1, NCKalpha, NCKNCK tyrosine kinase, non-catalytic region of tyrosine kinase, SH2/SH3 adaptor protein NCK-alpha

Gene Symbol

NCK1

Additional NCK1 Products

Product Documents for NCK1 Antibody (5B7) - BSA Free

Certificate of Analysis

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Product Specific Notices for NCK1 Antibody (5B7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Intracellular Kinases
PDGF Family

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Protocols

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FAQs

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Associated Pathways

VEGF - VEGF R2 Signaling Pathways VEGF - VEGF R2 Signaling Pathway Thumbnail