Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all NCOA2 Primary Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 1400 the C-terminus (residue 1464) of human Nuclear Receptor Coactivator 2 using the numbering given in entry NP_006531.1 (GeneID 10499).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NCOA2 Antibody - BSA Free

Western Blot: NCOA2 Antibody [NBP2-22162]

Western Blot: NCOA2 Antibody [NBP2-22162]

Western Blot: NCOA2 Antibody [NBP2-22162] - Western Blot: NCOA2 Antibody - hole cell lysate (50 ug) from TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified rabbit anti-NCOA2 used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry-Paraffin: NCOA2 Antibody [NBP2-22162]

Immunohistochemistry-Paraffin: NCOA2 Antibody [NBP2-22162]

Immunohistochemistry-Paraffin: NCOA2 Antibody [NBP2-22162] - Section of human testicular seminoma. Antibody: Affinity purified rabbit antiNCOA2/ SRC2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB.
Immunoprecipitation: NCOA2 Antibody [NBP2-22162]

Immunoprecipitation: NCOA2 Antibody [NBP2-22162]

Immunoprecipitation: NCOA2 Antibody [NBP2-22162] - Immunoprecipitation: NCOA2 Antibody - Nuclear extract (A, 10 mg) or cytosolic (S100) extract (B, 10 mg) from HeLa cells. Antibody: Affinity purified rabbit NCOA2 used at 20 ug/10 mg lysate. Detection: Coomassie Brilliant Blue R250 staining of SDS-PAGE gel followed by mass spectrometry to confirm the identity of the band that is NCOA2.

Applications for NCOA2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:500-1:2000

Immunoprecipitation

1-4ug/mg of Lysate

Western Blot

1:2000 - 1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NCOA2

Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcriptional activity of the nuclear receptor with which they associate. One such coactivator is Steroid Receptor Coactivator-2 (SRC2).

Long Name

Nuclear Receptor Co-activator 2

Alternate Names

bHLHe75, HTIF2, KAT13C, TIF2

Entrez Gene IDs

64708 (Human)

Gene Symbol

NCOA2

UniProt

Q9H9Q2 (Human)

Additional NCOA2 Products

Product Documents for NCOA2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for NCOA2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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