NDP52 Antibody (422) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-43696

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Validated:

Human

Predicted:

Chimpanzee (97%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 422

Format

Azide and BSA Free
View all NDP52 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human NDP52. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for NDP52 Antibody (422) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: NDP52 Antibody (422) [NBP2-43696]

Immunocytochemistry/ Immunofluorescence: NDP52 Antibody (422) [NBP2-43696]

Immunocytochemistry/Immunofluorescence: NDP52 Antibody (422) [NBP2-43696] - NDP52 antibody (422) detects NDP52 protein at autophagosome by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NDP52 stained by NDP52 antibody (422) diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Fluoroshield with DAPI. Scale bar= 10 um.
Western Blot: NDP52 Antibody (422) [NBP2-43696]

Western Blot: NDP52 Antibody (422) [NBP2-43696]

Western Blot: NDP52 Antibody (422) [NBP2-43696] - Analysis of A. 30 ug Huh7 whole cell lysate/extract B. 30 ug Hep3B whole cell lysate/extract C. 30 ug HepG2 whole cell lysate/extract 10 % SDS-PAGE NDP52 antibody [422] dilution: 1:1000
Western Blot: NDP52 Antibody (422) [NBP2-43696]

Western Blot: NDP52 Antibody (422) [NBP2-43696]

Western Blot: NDP52 Antibody (422) [NBP2-43696] - Analysis of A. 30 ug Jurkat whole cell lysate/extract B. 30 ug Raji whole cell lysate/extract C. 30 ug NCI-H929 whole cell lysate/extract 10 % SDS-PAGE NDP52 antibody [422] dilution: 1:1000.
Western Blot: NDP52 Antibody (422) [NBP2-43696]

Western Blot: NDP52 Antibody (422) [NBP2-43696]

Western Blot: NDP52 Antibody (422) [NBP2-43696] - Analysis of un-treated (-) and treated (+), Thapsigargin treatment for 12hrs and 24hrs. Huh-7 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody. ACTB was used as internal control (bottom panel).
Western Blot: NDP52 Antibody (422) [NBP2-43696]

Western Blot: NDP52 Antibody (422) [NBP2-43696]

Western Blot: NDP52 Antibody (422) [NBP2-43696] - Analysis of un-treated (-) and treated (+), Thapsigargin treatment for 12hrs. HepG2 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody. ACTB was used as internal control (bottom panel).
Immunocytochemistry/ Immunofluorescence: NDP52 Antibody (422) [NBP2-43696]

Immunocytochemistry/ Immunofluorescence: NDP52 Antibody (422) [NBP2-43696]

Immunocytochemistry/Immunofluorescence: NDP52 Antibody (422) [NBP2-43696] - Analysis of Samples: HeLa cells mock (left) and treated with 50uM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: NDP52 protein stained by NDP52 antibody [422]. Red: Phalloidin, a F-actin marker.
Immunocytochemistry/ Immunofluorescence: NDP52 Antibody (422) [NBP2-43696]

Immunocytochemistry/ Immunofluorescence: NDP52 Antibody (422) [NBP2-43696]

Immunocytochemistry/Immunofluorescence: NDP52 Antibody (422) [NBP2-43696] - Analysis of Samples: HepG2 cells mock (left) and treated with 3 uM Thapsigargin for 16 hrs (right) were fixed in ice-cold MeOH for 10 min and permeabilized with 100% MeOH for 30 sec. Green: NDP52 protein stained by NDP52 antibody [422]. Blue: Hoechst 33342 staining. Scale bar = 10 um.
Immunoprecipitation: NDP52 Antibody (422) [NBP2-43696]

Immunoprecipitation: NDP52 Antibody (422) [NBP2-43696]

Immunoprecipitation: NDP52 Antibody (422) [NBP2-43696] - Immunoprecipitation of NDP52 protein from Jurkat whole cell extracts using 5 ug of NDP52 antibody [422]. Western blot analysis was performed using NDP52 antibody [422]. EasyBlot anti-Mouse IgG was used as a secondary reagent.
NDP52 Antibody (422) - Azide and BSA Free

Western Blot: NDP52 Antibody (422) [NBP2-43696] -

Non-transfected (-) and transfected (+) HepG2 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody [GT422] (NBP2-43696) diluted at 1:1000.

Applications for NDP52 Antibody (422) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NDP52

NDP52 is encoded by this gene is a subunit of nuclear domain 10 (ND10) bodies. ND10 bodies are nuclear domains appearing immunohistochemically as ten dots per nucleus. They are believed to be associated with the nuclear matrix on the basis of their resistance to nuclease digestion and salt extraction. ND10 proteins are removed from the nucleus by herpes simplex virus-1 infection and may have a role in viral life cycles. [provided by RefSeq]

Alternate Names

Antigen nuclear dot 52 kDa protein, calcium binding and coiled-coil domain 2, calcium-binding and coiled-coil domain-containing protein 2, NDP52MGC17318, Nuclear domain 10 protein 52, Nuclear domain 10 protein NDP52, Nuclear dot protein 52

Gene Symbol

CALCOCO2

Additional NDP52 Products

Product Documents for NDP52 Antibody (422) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for NDP52 Antibody (422) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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