Ndufs4 Antibody (6T10F4)

Novus Biologicals | Catalog # NBP3-16679

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6T10F4 expressed in HEK293
View all Ndufs4 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human NDUFS4 (O43181). MAAVSMSVVLRQTLWRRRAVAVAALSVSRVPTRSLRTSTWRLAQDQTQDTQLITVDEKLDITTLTGVPEEHIKTRKVRIFVPARNNMQSGVNNTKKWKME

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Ndufs4 Antibody (6T10F4)

Western Blot: Ndufs4 Antibody (6T10F4) [NBP3-16679]

Western Blot: Ndufs4 Antibody (6T10F4) [NBP3-16679]

Western Blot: Ndufs4 Antibody (6T10F4) [NBP3-16679] - Western blot analysis of extracts of various cell lines, using Ndufs4 Rabbit mAb (NBP3-16679) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunocytochemistry/ Immunofluorescence: Ndufs4 Antibody (6T10F4) [NBP3-16679]

Immunocytochemistry/ Immunofluorescence: Ndufs4 Antibody (6T10F4) [NBP3-16679]

Immunocytochemistry/Immunofluorescence: Ndufs4 Antibody (6T10F4) [NBP3-16679] - Immunofluorescence analysis of NIH-3T3 cells using Ndufs4 Rabbit mAb (NBP3-16679) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blot: Ndufs4 Antibody (6T10F4) [NBP3-16679]

Western Blot: Ndufs4 Antibody (6T10F4) [NBP3-16679]

Western Blot: Ndufs4 Antibody (6T10F4) [NBP3-16679] - Western blot analysis of extracts of HepG2 cells, using Ndufs4 Rabbit mAb (NBP3-16679) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunocytochemistry/ Immunofluorescence: Ndufs4 Antibody (6T10F4) [NBP3-16679]

Immunocytochemistry/ Immunofluorescence: Ndufs4 Antibody (6T10F4) [NBP3-16679]

Immunocytochemistry/Immunofluorescence: Ndufs4 Antibody (6T10F4) [NBP3-16679] - Immunofluorescence analysis of C6 cells using Ndufs4 Rabbit mAb (NBP3-16679) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Ndufs4 Antibody (6T10F4)

Immunohistochemistry-Paraffin: Ndufs4 Antibody (6T10F4) [NBP3-16679] -

Immunohistochemistry-Paraffin: Ndufs4 Antibody (6T10F4) [NBP3-16679] - Analysis of NDUFS4 in paraffin-embedded human colon carcinoma tissue using NDUFS4 Rabbit mAb at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Ndufs4 Antibody (6T10F4)

Immunohistochemistry-Paraffin: Ndufs4 Antibody (6T10F4) [NBP3-16679] -

Immunohistochemistry-Paraffin: Ndufs4 Antibody (6T10F4) [NBP3-16679] - Analysis of NDUFS4 in paraffin-embedded human liver tissue using NDUFS4 Rabbit mAb at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Applications for Ndufs4 Antibody (6T10F4)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Ndufs4

Ndufs4 encodes an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), or NADH:ubiquinone oxidoreductase, the first multi-subunit enzyme complex of the mitochondrial respiratory chain. Complex I plays a vital role in cellular ATP production, the primary source of energy for many crucial processes in living cells. It removes electrons from NADH and passes them by a series of different protein-coupled redox centers to the electron acceptor ubiquinone. In well-coupled mitochondria, the electron flux leads to ATP generation via the building of a proton gradient across the inner membrane. Complex I is composed of at least 41 subunits, of which 7 are encoded by the mitochondrial genome and the remainder by nuclear genes.

Alternate Names

AQDQ, AQDQmitochondrial, CI-18 kDa, NADH dehydrogenase (ubiquinone) Fe-S protein 4 (18kD) (NADH-coenzyme Qreductase), NADH dehydrogenase (ubiquinone) Fe-S protein 4, 18kDa (NADH-coenzyme Qreductase), NADH-coenzyme Q reductase, 18-KD, NADH-ubiquinone oxidoreductase 18 kDa subunit

Gene Symbol

NDUFS4

Additional Ndufs4 Products

Product Documents for Ndufs4 Antibody (6T10F4)

Certificate of Analysis

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Product Specific Notices for Ndufs4 Antibody (6T10F4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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