NDUFV1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-33074

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Zebrafish

Cited:

Mouse

Predicted:

Bovine (98%), Chicken (95%), Chimpanzee (100%), Rat (97%), Xenopus (90%). Backed by our 100% Guarantee.

Applications

Validated:

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human NDUFV1. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (90%).

Localization

Mitochondrion inner membrane, Peripheral membrane protein, Matrix side

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NDUFV1 Antibody - BSA Free

Western Blot: NDUFV1 Antibody [NBP1-33074]

Western Blot: NDUFV1 Antibody [NBP1-33074]

Western Blot: NDUFV1 Antibody [NBP1-33074] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NDUFV1 antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: NDUFV1 Antibody [NBP1-33074]

Immunohistochemistry-Paraffin: NDUFV1 Antibody [NBP1-33074]

Immunohistochemistry-Paraffin: NDUFV1 Antibody [NBP1-33074] - Analysis of paraffin-embedded zebrafish tissue, using NDUFV1 antibody [N3C3] at 1:300 dilution.
Western Blot: NDUFV1 Antibody [NBP1-33074]

Western Blot: NDUFV1 Antibody [NBP1-33074]

Western Blot: NDUFV1 Antibody [NBP1-33074] - Whole zebrafish extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with NDUFV1 antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: NDUFV1 Antibody [NBP1-33074]

Immunohistochemistry-Paraffin: NDUFV1 Antibody [NBP1-33074]

Immunohistochemistry-Paraffin: NDUFV1 Antibody [NBP1-33074] - U373 xenograft, using NDUFV1 antibody at 1:500 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Immunohistochemistry-Paraffin: NDUFV1 Antibody [NBP1-33074]

Immunohistochemistry-Paraffin: NDUFV1 Antibody [NBP1-33074]

Immunohistochemistry-Paraffin: NDUFV1 Antibody [NBP1-33074] - Mouse kidney. NDUFV1 antibody [N3C3] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Immunohistochemistry-Paraffin: NDUFV1 Antibody [NBP1-33074]

Immunohistochemistry-Paraffin: NDUFV1 Antibody [NBP1-33074]

Immunohistochemistry-Paraffin: NDUFV1 Antibody [NBP1-33074] - Mouse kidney. NDUFV1 antibody [N3C3] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
NDUFV1 Antibody

Western Blot: NDUFV1 Antibody [NBP1-33074] -

Western Blot: NDUFV1 Antibody [NBP1-33074] - Wild-type (WT) and NDUFV1 knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NDUFV1 antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for NDUFV1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NDUFV1

The NDUFV1 gene encodes the 51-kD subunit of complex I (NADH:ubiquinone oxidoreductase) of the mitochondrial respiratory chain.[supplied by OMIM]

Alternate Names

CI-51K, CI51KD, CI-51kD, complex I 51kDa subunit, complex I, mitochondrial respiratory chain, Complex I-51kD, EC 1.6.5.3, EC 1.6.99.3, EC 1.6.99.5, FLJ59059, mitochondrial NADH dehydrogenase ubiquinone flavoprotein 1, NADH dehydrogenase (ubiquinone) flavoprotein 1 (51kD), NADH dehydrogenase (ubiquinone) flavoprotein 1, 51kDa, NADH dehydrogenase flavoprotein 1, NADH-ubiquinone oxidoreductase 51 kDa subunit, UQOR1NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial

Gene Symbol

NDUFV1

Additional NDUFV1 Products

Product Documents for NDUFV1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for NDUFV1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for NDUFV1 Antibody - BSA Free

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Protocols

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