Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI6F8

Format

Azide and BSA Free
View all Nectin-1 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PVRL1(NP_002846) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse Nectin-1/PVRL1 Antibody (OTI6F8) - Azide and BSA Free (NBP2-01198) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Nectin-1/PVRL1 Antibody (OTI6F8) - Azide and BSA Free

Western Blot: Nectin-1/PVRL1 Antibody (OTI6F8)Azide and BSA Free [NBP2-72929]

Western Blot: Nectin-1/PVRL1 Antibody (OTI6F8)Azide and BSA Free [NBP2-72929]

Western Blot: Nectin-1/PVRL1 Antibody (OTI6F8) - Azide and BSA Free [NBP2-72929] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PVRL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PVRL1.
Immunocytochemistry/ Immunofluorescence: Nectin-1/PVRL1 Antibody (OTI6F8) - Azide and BSA Free [NBP2-72929]

Immunocytochemistry/ Immunofluorescence: Nectin-1/PVRL1 Antibody (OTI6F8) - Azide and BSA Free [NBP2-72929]

Immunocytochemistry/Immunofluorescence: Nectin-1/PVRL1 Antibody (OTI6F8) - Azide and BSA Free [NBP2-72929] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PVRL1.
Immunohistochemistry: Nectin-1/PVRL1 Antibody (OTI6F8) - Azide and BSA Free [NBP2-72929]

Immunohistochemistry: Nectin-1/PVRL1 Antibody (OTI6F8) - Azide and BSA Free [NBP2-72929]

Immunohistochemistry: Nectin-1/PVRL1 Antibody (OTI6F8) - Azide and BSA Free [NBP2-72929] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PVRL1 mouse monoclonal antibody.
Immunohistochemistry: Nectin-1/PVRL1 Antibody (OTI6F8) - Azide and BSA Free [NBP2-72929]

Immunohistochemistry: Nectin-1/PVRL1 Antibody (OTI6F8) - Azide and BSA Free [NBP2-72929]

Immunohistochemistry: Nectin-1/PVRL1 Antibody (OTI6F8) - Azide and BSA Free [NBP2-72929] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PVRL1 mouse monoclonal antibody.

Applications for Nectin-1/PVRL1 Antibody (OTI6F8) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Nectin-1

The PVRL1 gene encodes an adhesion protein that plays a role in the organization of adherens junctions and tight junctions in epithelial and endothelial cells. The protein is a calcium(2+)-independent cell-cell adhesion molecule that belongs to the immunoglobu

Long Name

Poliovirus Receptor Related 1

Alternate Names

CD111, HVEC, Nectin1, PRR1, PVRL1, PVRR1

Gene Symbol

NECTIN1

Additional Nectin-1 Products

Product Documents for Nectin-1/PVRL1 Antibody (OTI6F8) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Nectin-1/PVRL1 Antibody (OTI6F8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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