Nectin-1/PVRL1 Antibody (R1.302) - BSA Free

Novus Biologicals | Catalog # NBP2-54643

Clone R1.302 was used by HLDA to establish CD designation.
Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Immunohistochemistry, Western Blot, Flow Cytometry, Flow (Cell Surface), Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # R1.302

Format

BSA Free
View all Nectin-1 Primary Antibodies »

Product Specifications

Immunogen

NIH/3T3 cells transfected with human CD111

Specificity

The mouse monoclonal antibody R1.302 recognizes CD111 (also known as Nectin 1), a 75 kDa type I transmembrane glycoprotein broadly expressed on endothelial cells, epithelial cells, neuronal cells, megakaryocytes, and CD34-positive stem cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

75 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Nectin-1/PVRL1 Antibody (R1.302) - BSA Free

Flow (Cell Surface): Nectin-1/PVRL1 Antibody (R1.302) - BSA Free [NBP2-54643]

Flow (Cell Surface): Nectin-1/PVRL1 Antibody (R1.302) - BSA Free [NBP2-54643]

Flow (Cell Surface): Nectin-1/PVRL1 Antibody (R1.302) [NBP2-54643] - Surface staining of human peripheral blood with anti-human CD111 (R1.302) PE.
Flow Cytometry: Nectin-1/PVRL1 Antibody (R1.302) - BSA Free [NBP2-54643]

Flow Cytometry: Nectin-1/PVRL1 Antibody (R1.302) - BSA Free [NBP2-54643]

Flow Cytometry: Nectin-1/PVRL1 Antibody (R1.302) [NBP2-54643] - Surface staining of human peripheral blood with anti-human CD111 (R1.302) purified, GAM-APC.

Applications for Nectin-1/PVRL1 Antibody (R1.302) - BSA Free

Application
Recommended Usage

Flow (Cell Surface)

1:10-1:1000

Flow Cytometry

1-4 ug/ml
Application Notes
This antibody is Cytof ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nectin-1

CD111, also known as nectin-1, is a calcium-independent cell-cell adhesion transmembrane glycoprotein involved in organization of adherens junctions and tight junctions in epithelial and endothelial cells. It also serves as a target molecule for entry of herpes simplex virus (HSV-1, HSV-2) and pseudorabies virus (PRV) into epithelial and neuronal cells. CD111 is connected with actin cytoskeleton through afadin. Mutations in the gene for CD111 cause cleft lip and palate/ectodermal dysplasia 1 syndrome (CLPED1) as well as non-syndromic cleft lip with or without cleft palate (CL/P). Alternative splicing results in multiple transcript variants encoding proteins with distinct C-termini.

Long Name

Poliovirus Receptor Related 1

Alternate Names

CD111, HVEC, Nectin1, PRR1, PVRL1, PVRR1

Gene Symbol

NECTIN1

Additional Nectin-1 Products

Product Documents for Nectin-1/PVRL1 Antibody (R1.302) - BSA Free

Certificate of Analysis

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Product Specific Notices for Nectin-1/PVRL1 Antibody (R1.302) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Nectin-1/PVRL1 Antibody (R1.302) - BSA Free

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Protocols

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FAQs

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