Nectin-2/CD112 Antibody (S06-8F3)
Novus Biologicals | Catalog # NBP3-20070
Recombinant Monoclonal Antibody
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # S06-8F3 expressed in HEK293
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Product Specifications
Immunogen
A synthetic peptide of human Nectin-2/CD112 (Uniprot # Q92692)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Nectin-2/CD112 Antibody (S06-8F3)
Immunocytochemistry/ Immunofluorescence: Nectin-2/CD112 Antibody (S06-8F3) [NBP3-20070] -
Immunocytochemistry/ Immunofluorescence: Nectin-2/CD112 Antibody (S06-8F3) [NBP3-20070] - Immunocytochemistry of Nectin-2/CD112 (green) in hela using NBP3-20070 at dilution 1/50, and DAPI(blue)
Western Blot: Nectin-2/CD112 Antibody (S06-8F3) [NBP3-20070] -
Western Blot: Nectin-2/CD112 Antibody (S06-8F3) [NBP3-20070] - Western blot detection of Hela, A549, HL-60, U251, U87-MG cell lysates using NBP3-20070. Predicted band size: 58kDa. Observed band size: 70-80kDa.Applications for Nectin-2/CD112 Antibody (S06-8F3)
Application
Recommended Usage
Flow Cytometry
0.0763888888888889
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Immunoprecipitation
1:100
Western Blot
1:500-1:2000
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.4), 150mM NaCl, 50% glycerol.
Preservative
0.02% Sodium Azide
Concentration
0.3 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Nectin-2/CD112
Long Name
Poliovirus Receptor Related 2
Alternate Names
CD112, HVEB, MPH, Nectin2, PRR2, PVRL2
Gene Symbol
NECTIN2
Additional Nectin-2/CD112 Products
Product Documents for Nectin-2/CD112 Antibody (S06-8F3)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Nectin-2/CD112 Antibody (S06-8F3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunoprecipitation Protocol
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Liperfluo
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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