NEIL1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-47358

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all NEIL1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: KSKATQLSPEDRVEDALPPSKAPSRTRRAKRDLPKRTATQRPEGTSLQQDPEAPTVPKKGRRKGRQAASGHCRPRKVKADIPSLEPE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NEIL1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: NEIL1 Antibody [NBP2-47358]

Immunocytochemistry/ Immunofluorescence: NEIL1 Antibody [NBP2-47358]

Immunocytochemistry/Immunofluorescence: NEIL1 Antibody [NBP2-47358] - Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: NEIL1 Antibody [NBP2-47358]

Immunohistochemistry-Paraffin: NEIL1 Antibody [NBP2-47358]

Immunohistochemistry-Paraffin: NEIL1 Antibody [NBP2-47358] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: NEIL1 Antibody [NBP2-47358]

Immunohistochemistry-Paraffin: NEIL1 Antibody [NBP2-47358]

Immunohistochemistry-Paraffin: NEIL1 Antibody [NBP2-47358] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.
Immunohistochemistry-Paraffin: NEIL1 Antibody [NBP2-47358]

Immunohistochemistry-Paraffin: NEIL1 Antibody [NBP2-47358]

Immunohistochemistry-Paraffin: NEIL1 Antibody [NBP2-47358] - Staining of human fallopian tube shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: NEIL1 Antibody [NBP2-47358]

Immunohistochemistry-Paraffin: NEIL1 Antibody [NBP2-47358]

Immunohistochemistry-Paraffin: NEIL1 Antibody [NBP2-47358] - Staining of human lymphoid tissues shows strong nuclear positivity in germinal center cells.

Applications for NEIL1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NEIL1

NEIL1 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction (Bandaru et al., 2002).[supplied by OMIM]

Alternate Names

DNA-(apurinic or apyrimidinic site) lyase Neil1, EC 3.2.2.-, EC 4.2.99.18, endonuclease 8-like 1, endonuclease VIII, Endonuclease VIII-like 1, FLJ22402, FPG1DNA glycosylase/AP lyase Neil1, hFPG1, NEH1, nei endonuclease VIII-like 1 (E. coli), Nei homolog 1, NEI1, Nei-like protein 1

Gene Symbol

NEIL1

Additional NEIL1 Products

Product Documents for NEIL1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for NEIL1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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