NEK2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-78022

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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all NEK2 Primary Antibodies »

Product Specifications

Immunogen

This affinity purified NEK2 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 275-300 of Human NEK2 protein. (Uniprot: P51955)

Reactivity Notes

BLAST analysis indicates 100 % homology of the immunizing sequence with NEK2 homologues from human and chimpanzee. Cross reactivity with NEK2 protein homologues from other sources may not occur as sequence homology varies by at least one amino acid residue in this sequence.

Specificity

BLAST analysis indicates 100 % homology of the immunizing sequence with NEK2 homologues from human and chimpanzee. Cross reactivity with NEK2 protein homologues from other sources may not occur as sequence homology varies by at least one amino acid residue in this sequence.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This product is an affinity-purified antibody produced by immunoaffinity chromatography using peptide coupled to agarose beads

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for NEK2 Antibody - BSA Free

Western Blot: NEK2 Antibody [NBP1-78022]

Western Blot: NEK2 Antibody [NBP1-78022]

Western Blot: NEK2 Antibody [NBP1-78022] - Western blot analysis is shown using Affinity Purified NEK2 antibody to detect enCanineenous protein present in an unstimulated mouse A-20 whole cell lysate (arrowhead). Comparison to a molecular weight marker indicates a band of ~52 kDa correspond-ing to NEK2 protein. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using standard techniques.
Western Blot: NEK2 Antibody [NBP1-78022]

Western Blot: NEK2 Antibody [NBP1-78022]

Western Blot: NEK2 Antibody [NBP1-78022] - Western Blot of NEK2 antibody. Lane 1: Opal Pre-stained Molecular Weight Marker. Lane 2: Jurkat cell lysate (10ug) spiked with rec. NEK2 protein (50ng). Lane 3: Jurkat cell lysate (10ug) spiked with rec. NEK2 protein (20ng). Lane 4: Jurkat cell lysate (10ug) spiked with rec. NEK2 protein (20ng). Lane 5: Jurkat cell lysate (10ug). Primary Antibody: Anti-NEK2 at 1ug/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:70,000 for 30mins at RT. Block: BlockOut Buffer. Expect/Observed MW: ~76kDa rec NEK2.
N/A: NEK2 Antibody [NBP1-78022]

Applications for NEK2 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:2000-1:10000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Western Blot

1:500-1:2000
Application Notes
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 52 kDa in size corresponding to NEK2 protein by western blotting in the appropriate cell lysate or extract. Splice variants exist for this protein that may result in the detection of lower molecular weight bands.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: NEK2

NEK2, also called serine/threonine-protein kinase Nek2NimA-related protein kinase 2, NimA-like protein kinase 1 and HSPK 21, is a protein kinase involved in mitotic regulation that may have a role at the G2-M transition and may also play a role in meiosis. NEK2 has a nuclear localization and accumulates throughout S phase and shows maximal levels in late G2. This expression pattern is highly reminiscent of that of A and B cyclins.

Long Name

NIMA [Never In Mitosis Gene A]-related Kinase 2

Alternate Names

HsPK21, NLK1

Gene Symbol

NEK2

UniProt

NC_000001.10 (Human), P51955 (Human)

Additional NEK2 Products

Product Documents for NEK2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for NEK2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers
Intracellular Kinases

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Protocols

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