NEK2 Antibody

Novus Biologicals | Catalog # NBP2-19510

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all NEK2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein corresponding to human NEK2.

Localization

Nucleus; Isoform 1: Nucleus; nucleolus; Isoform 2: Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NEK2 Antibody

Western Blot: NEK2 Antibody [NBP2-19510]

Western Blot: NEK2 Antibody [NBP2-19510]

Western Blot: NEK2 Antibody [NBP2-19510] - Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: NEK2 Antibody [NBP2-19510]

Immunocytochemistry/ Immunofluorescence: NEK2 Antibody [NBP2-19510]

Immunocytochemistry/Immunofluorescence: NEK2 Antibody [NBP2-19510] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NEK2 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.
Immunohistochemistry-Paraffin: NEK2 Antibody [NBP2-19510]

Immunohistochemistry-Paraffin: NEK2 Antibody [NBP2-19510]

Immunohistochemistry-Paraffin: NEK2 Antibody [NBP2-19510] - Paraffin-embedded human ovarian carcinoma. NEK2 antibody diluted at 1:250.
NEK2 Antibody

Western Blot: NEK2 Antibody [NBP2-19510] -

Western Blot: NEK2 Antibody [NBP2-19510] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NEK2 antibody (NBP2-19510) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for NEK2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NEK2

Protein kinase that is involved in mitotic regulation. Integral component of the mitotic spindle-assembly checkpoint which is necessary for proper chromosome segregation during metaphase-anaphase transition. Required for association of MAD2L1 to kinetochore. Phosphorylates SGOL1. May have a role at the G2-M transition. May also play a role in meiosis. Isoform 1 but not isoform 2 appears to play a role in centrosome splitting. Isoform 1 phosphorylates and activates NEK11 in G1/S-arrested cells. Isoform 2, which is not present in the nucleolus, does not.

Long Name

NIMA [Never In Mitosis Gene A]-related Kinase 2

Alternate Names

HsPK21, NLK1

Gene Symbol

NEK2

Additional NEK2 Products

Product Documents for NEK2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for NEK2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Cancer Biomarkers
Intracellular Kinases

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Protocols

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