Neprilysin/CD10 Antibody (FR4D11)

Novus Biologicals | Catalog # NBP2-44868

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # FR4D11
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Product Specifications

Immunogen

Raji cells

Localization

Cell Surface

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-11515)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Neprilysin/CD10 Antibody (FR4D11)

Flow Cytometry: Neprilysin/CD10 Antibody (FR4D11) [NBP2-44868]

Flow Cytometry: Neprilysin/CD10 Antibody (FR4D11) [NBP2-44868]

Flow Cytometry: Neprilysin/CD10 Antibody (FR4D11) [NBP2-44868] - Flow Cytometric Analysis of Ramos cells using Neprilysin/CD10 Antibody (FR4D11) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).
Immunofluorescence: Neprilysin/CD10 Antibody (FR4D11) [NBP2-44868]

Immunofluorescence: Neprilysin/CD10 Antibody (FR4D11) [NBP2-44868]

Immunofluorescence: Neprilysin/CD10 Antibody (FR4D11) [NBP2-44868] - ICC/IF staining of Ramos cells using followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Red Dot.
Neprilysin/CD10 Antibody (FR4D11) Immunocytochemistry/ Immunofluorescence: Neprilysin/CD10 Antibody (FR4D11) [NBP2-44868] -

Immunocytochemistry/ Immunofluorescence: Neprilysin/CD10 Antibody (FR4D11) [NBP2-44868] -

Immunofluorescence staining of Ramos cells using Neprilysin/CD10 Antibody (FR4D11) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.

Applications for Neprilysin/CD10 Antibody (FR4D11)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.

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Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Neprilysin/CD10

CD10, also known as the Common Acute Lymphocytic Leukemia Antigen (CALLA), is a cell surface enzyme with neutral metalloendopeptidase activity which inactivates a variety of biologically active peptides. CD10 is expressed on the cells of lymphoblastic, Burkitt's, and follicular germinal center lymphomas, immature B cells with in adult bone marrow and on cells from patients with chronic myelocytic leukemia (CML). CD10 is also present on breast myoepithelial cells, bile canaliculi, fibroblasts, with especially high expression on the brush border of kidney and gut epithelial cells.

Alternate Names

CALLA, CD10, Enkephalinase, Leu-19, MME, Neutral Endopeptidase 24.11, NKH1

Gene Symbol

MME

UniProt

Additional Neprilysin/CD10 Products

Product Documents for Neprilysin/CD10 Antibody (FR4D11)

Certificate of Analysis

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Product Specific Notices for Neprilysin/CD10 Antibody (FR4D11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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