Nesprin 2 Antibody (10H8) - BSA Free

Novus Biologicals | Catalog # NBP2-59945

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 10H8

Format

BSA Free
View all Nesprin 2 Primary Antibodies »

Product Specifications

Immunogen

GST fused to residues 6,653-6,835 of human Nesprin 2

Specificity

Specificity of human Nesprin 2 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for Nesprin 2 Antibody (10H8) - BSA Free

Western Blot: Nesprin 2 Antibody (10H8)BSA Free [NBP2-59945]

Western Blot: Nesprin 2 Antibody (10H8)BSA Free [NBP2-59945]

Western Blot: Nesprin 2 Antibody (10H8) [NBP2-59945] - HEK293 cells transiently expressing either GFP alone or GFP fused a small Nesp2 isoform (Nesp2a2DKASH) lacking the C-terminal KASH domain. This small isoform contains the ~180 residue Nesp2 polypeptide that was employed in the immunogen. Total cell lysates were analyzed by western blot employing either a rabbit anti-GFP or culture supernatant from three independent anti-Nesp2 hybridoma clones (6C11, 10H8 and 5C3)
Immunocytochemistry/ Immunofluorescence: Nesprin 2 Antibody (10H8) - BSA Free [NBP2-59945]

Immunocytochemistry/ Immunofluorescence: Nesprin 2 Antibody (10H8) - BSA Free [NBP2-59945]

Immunocytochemistry/Immunofluorescence: Nesprin 2 Antibody (10H8) [NBP2-59945] - Staining in HeLa cells labeled with either 6C11 or 10H8 monoclonal antibodies raised against human Nesp2. The antibodies can be used on either formaldehyde- or methanol-fixed cells.

Applications for Nesprin 2 Antibody (10H8) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Western Blot

1:100 - 1-2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nesprin 2

SYNE2 - spectrin repeat containing, nuclear envelope 2

Alternate Names

DKFZP434H2235, DKFZp686E01115, DKFZp686H1931, EDMD5, FLJ46790, KIAA1011FLJ11014, nesprin 2, nesprin-2, NUAFLJ43727, NUANCEFLJ45710, Nuclear envelope spectrin repeat protein 2, Nucleus and actin connecting element protein, polytrophin, Protein NUANCE, spectrin repeat containing, nuclear envelope 2, Synaptic nuclear envelope protein 2, synaptic nuclei expressed gene 2, SYNE-2, TROPH

Gene Symbol

SYNE2

Additional Nesprin 2 Products

Product Documents for Nesprin 2 Antibody (10H8) - BSA Free

Certificate of Analysis

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Product Specific Notices for Nesprin 2 Antibody (10H8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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