NEU2 Antibody (OTI4F4) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-72944

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI4F4

Format

Azide and BSA Free
View all NEU2 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human NEU2 (NP_005374) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

42.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NEU2 Antibody (OTI4F4) - Azide and BSA Free

Western Blot: NEU2 Antibody (OTI4F4)Azide and BSA Free [NBP2-72944]

Western Blot: NEU2 Antibody (OTI4F4)Azide and BSA Free [NBP2-72944]

Western Blot: NEU2 Antibody (OTI4F4) - Azide and BSA Free [NBP2-72944] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEU2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEU2.
Immunocytochemistry/ Immunofluorescence: NEU2 Antibody (OTI4F4) - Azide and BSA Free [NBP2-72944]

Immunocytochemistry/ Immunofluorescence: NEU2 Antibody (OTI4F4) - Azide and BSA Free [NBP2-72944]

Immunocytochemistry/Immunofluorescence: NEU2 Antibody (OTI4F4) - Azide and BSA Free [NBP2-72944] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY NEU2.
Flow Cytometry: NEU2 Antibody (OTI4F4) - Azide and BSA Free [NBP2-72944]

Flow Cytometry: NEU2 Antibody (OTI4F4) - Azide and BSA Free [NBP2-72944]

Flow Cytometry: NEU2 Antibody (OTI4F4) - Azide and BSA Free [NBP2-72944] - Analysis of Hela cells, using anti-NEU2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Applications for NEU2 Antibody (OTI4F4) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:2000

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NEU2

NEU2 belongs to a family of glycohydrolytic enzymes which remove sialic acid residues from glycoproteins and glycolipids. Expression studies in COS7 cells confirmed that this gene encodes a functional sialidase. Its cytosolic localization was demonstrated by cell fractionation experiments.

Long Name

Sialidase-2

Alternate Names

Cytosolic sialidase, EC 3.2.1.18

Gene Symbol

NEU2

Additional NEU2 Products

Product Documents for NEU2 Antibody (OTI4F4) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for NEU2 Antibody (OTI4F4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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