Neurogenin-1 Antibody (OTI3F9)

Novus Biologicals | Catalog # NBP1-47871

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3F9
View all Neurogenin-1 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human NEUROG1 (NP_006152) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This antibody is specific for Homo sapiens neurogenin 1 (NEUROG1).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

25.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Neurogenin-1 Antibody (OTI3F9)

Western Blot: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871]

Western Blot: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871]

Western Blot: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Neurogenin 1 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871]

Immunocytochemistry/ Immunofluorescence: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871]

Immunocytochemistry/Immunofluorescence: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871] - COS7 cells transiently transfected by pCMV6-ENTRY Neurogenin-1.
Immunohistochemistry-Paraffin: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871]

Immunohistochemistry-Paraffin: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871]

Immunohistochemistry-Paraffin: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871] - Staining of Human prostate tissue.
Western Blot: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871]

Western Blot: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871]

Western Blot: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Neurogenin 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Neurogenin 1.
Immunohistochemistry-Paraffin: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871]

Immunohistochemistry-Paraffin: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871]

Immunohistochemistry-Paraffin: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871] - Staining of Human colon tissue.
Immunohistochemistry-Paraffin: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871]

Immunohistochemistry-Paraffin: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871]

Immunohistochemistry-Paraffin: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871] - Staining of Human Kidney tissue.
Immunohistochemistry-Paraffin: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871]

Immunohistochemistry-Paraffin: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871]

Immunohistochemistry-Paraffin: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871] - Staining of Human lung tissue.
Immunohistochemistry-Paraffin: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871]

Immunohistochemistry-Paraffin: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871]

Immunohistochemistry-Paraffin: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871] - Staining of Human Ovary tissue.
Immunohistochemistry-Paraffin: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871]

Immunohistochemistry-Paraffin: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871]

Immunohistochemistry-Paraffin: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871] - Staining of Human pancreas tissue.
Immunoprecipitation: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871]

Immunoprecipitation: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871]

Immunoprecipitation: Neurogenin-1 Antibody (OTI3F9) [NBP1-47871] - Immunoprecipitation (IP) of NEUROG1 by using TrueMab monoclonal anti-NEUROG1 antibodies (Negative control: IP without adding anti-NEUROG1 antibody.). For each experiment, 500ul of DDK tagged NEUROG1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-NEUROG1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Applications for Neurogenin-1 Antibody (OTI3F9)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Immunoprecipitation

2-4ug/ml

Western Blot

1:500-1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Neurogenin-1

Neurogenin 1 is the protein product of an activator-type bHLH gene (Ngn1). The activator-type bHLH genes (which also include Mash1 and Math) promote neuronal fate determination and induce expression of Notch ligands such as Delta which activate Notch signaling. The activator type bHLH genes are antagonized by repressor-type basic helix-loop-helix (bHLH) genes such as Hes1, 3 and 5. Thus, activator-type and repressor-type bHLH genes regulate each other, allowing only subsets of neural stem cells to undergo differentiation. Neurogenin 1 is thus one of the key regulators of neural stem cell differentiation, which is essential for generation of complex brain structures of appropriate size, shape and cell arrangement.

Alternate Names

AKA, Math4C, NEUROD3, NEUROG1, Neurogenin1

Entrez Gene IDs

4762 (Human)

Gene Symbol

NEUROG1

Additional Neurogenin-1 Products

Product Documents for Neurogenin-1 Antibody (OTI3F9)

Certificate of Analysis

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Product Specific Notices for Neurogenin-1 Antibody (OTI3F9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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