Newborn Calf Serum, Heat Inactivated
Please Note: An additional $10/bottle handling fee will be applied for 500 mL bottles.
Newborn Calf Serum, Heat Inactivated SummaryNewborn Calf Serum (NBC, NBCS) provides many of the same nutrients as Fetal Bovine Serum, but has slightly higher protein levels.
Why Use Newborn Calf Serum?
Newborn Bovine Calf Serum is collected from healthy, inspected bovine calves that are less than 14 days of age and manufactured under stringent collection and processing standards. It is used for routine maintenance of select cell lines and where low antibody concentrations are not critical. Newborn Calf Serum is often used as a lower-priced alternative to FBS.
Serum products should be stored and handled correctly to assure long-term stability and to preserve growth performance consistency throughout its shelf-life. In addition, heat inactivation of serum is frequently desired to inactivate complement within the serum. Below are protocol links for serum storage/handling and heat inactivation.
Protocol for Fetal Bovine Serum Storage, Thawing, and Freezing.
Protocol for Heat Inactivation of Serum Products.
Why Heat Inactivate Serum?
The objective of heat inactivation is to destroy complement activity in the serum without affecting the growth-promoting characteristics of the product. Removal of complement activity from the serum is not required for most cell cultures, but may be necessary for cultures that are sensitive to the complement activity. Since heat inactivation of the serum may, to some extent, decrease the growth performance properties of the serum, this procedure should only be performed if actually required for optimal cell growth. Researchers should evaluate the applicability of heat inactivation in regards to their own application. If heat inactivation is required, the process should be carefully controlled to avoid increased formation of crystalline and flocculent precipitates, gelling of serum proteins and excessive loss of growth performance. Significant damage to serum can occur when it is subjected to higher than required temperatures or heated over extended lengths of time.
For research use only. Not for diagnostic use.
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