NF-H Antibody (CL2671) - BSA Free

Novus Biologicals | Catalog # NBP2-46615

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Rat (94%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL2671

Format

BSA Free
View all NF-H Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P12036, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Expected to cross react based on sequence identity: Mouse (88%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for NF-H Antibody (CL2671) - BSA Free

Western Blot: NF-H Antibody (CL2671) [NBP2-46615]

Western Blot: NF-H Antibody (CL2671) [NBP2-46615]

Western Blot: NF-H Antibody (2671) [NBP2-46615] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue
Immunohistochemistry: NF-H Antibody (CL2671) [NBP2-46615]

Immunohistochemistry: NF-H Antibody (CL2671) [NBP2-46615]

Immunohistochemistry: NF-H Antibody (2671) [NBP2-46615] - Staining of human tonsil shows absence of immunoreactivity (negative control).
Immunohistochemistry: NF-H Antibody (CL2671) [NBP2-46615]

Immunohistochemistry: NF-H Antibody (CL2671) [NBP2-46615]

Immunohistochemistry: NF-H Antibody (2671) [NBP2-46615] - Staining of human cerebellum shows moderate immunoreactivity in Purkinje cells and neural fibers.
Immunohistochemistry: NF-H Antibody (CL2671) [NBP2-46615]

Immunohistochemistry: NF-H Antibody (CL2671) [NBP2-46615]

Immunohistochemistry: NF-H Antibody (2671) [NBP2-46615] - Staining of human cerebral cortex shows moderate positivity in neural fibers.

Applications for NF-H Antibody (CL2671) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NF-H

Neurofilaments are 10nm intermediate filament proteins located in vertebrate neurons, which regulate axonal diameter. They are composed predominantly of the three major neurofilament proteins: NF-Light, NF-Medium and NF-Heavy. NF-H is the heavy or high molecular weight microfilament subunit and runs on SDS-PAGE gels in the range 180-220kDa, with some size variation in across species.

Antibodies to NF-H may be used to identify NF-H in neurons and to study neuronal processes in tissue sections and/or culture. NF-H antibodies can also be used to study microfilament accumulations seen in many neurological diseases, such as Lou Geri's disease or Alzheimer's disease.

Long Name

Neurofilament Protein, Heavy Chain

Alternate Names

NEFH, NFH

Entrez Gene IDs

4744 (Human)

Gene Symbol

NEFH

UniProt

P12036

Additional NF-H Products

Product Documents for NF-H Antibody (CL2671) - BSA Free

Certificate of Analysis

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Product Specific Notices for NF-H Antibody (CL2671) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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