NF-H Antibody (rNF421)

Novus Biologicals | Catalog # NBP3-48940

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine, Chicken

Applications

Immunohistochemistry, Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 kappa Clone # rNF421 expressed in HEK293
View all NF-H Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length human NEFH protein (Uniprot: P12036)

Localization

Axon, Cell projection, Cytoplasm, Cytoskeleton

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

200 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Scientific Data Images for NF-H Antibody (rNF421)

NF-H Antibody (rNF421)

Western Blot: NF-H Antibody (rNF421) [NBP3-48940] -

Western Blot Analysis of human brain tissue lysate using NF-H Antibody (rNF421).
NF-H Antibody (rNF421)

Flow Cytometry: NF-H Antibody (rNF421) [NBP3-48940] -

Flow Cytometric Analysis of HEK293 cells using NF-H Antibody (rNF421) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).
NF-H Antibody (rNF421)

Immunohistochemistry-Paraffin: NF-H Antibody (rNF421) [NBP3-48940] -

Formalin-fixed, paraffin-embedded human Brain stained with NF-H Antibody (rNF421).

Applications for NF-H Antibody (rNF421)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: NF-H

Neurofilaments are 10nm intermediate filament proteins located in vertebrate neurons, which regulate axonal diameter. They are composed predominantly of the three major neurofilament proteins: NF-Light, NF-Medium and NF-Heavy. NF-H is the heavy or high molecular weight microfilament subunit and runs on SDS-PAGE gels in the range 180-220kDa, with some size variation in across species.

Antibodies to NF-H may be used to identify NF-H in neurons and to study neuronal processes in tissue sections and/or culture. NF-H antibodies can also be used to study microfilament accumulations seen in many neurological diseases, such as Lou Geri's disease or Alzheimer's disease.

Long Name

Neurofilament Protein, Heavy Chain

Alternate Names

NEFH, NFH

Gene Symbol

NEFH

Additional NF-H Products

Product Documents for NF-H Antibody (rNF421)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for NF-H Antibody (rNF421)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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