NF-L Antibody (CL4729) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-44128

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL4729

Format

Azide and BSA Free
View all NF-L Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P07196, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for NF-L Antibody (CL4729) - Azide and BSA Free

NF-L Antibody (CL4729) - Azide and BSA Free Immunohistochemistry-Paraffin: NF-L Antibody [NBP3-44128]

Immunohistochemistry-Paraffin: NF-L Antibody [NBP3-44128]

Staining of human cerebellum shows strong cytoplasmic immunoreactivity in cell bodies and proximal dendrites of Purkinje cells.
NF-L Antibody (CL4729) - Azide and BSA Free Immunohistochemistry-Paraffin: NF-L Antibody [NBP3-44128]

Immunohistochemistry-Paraffin: NF-L Antibody [NBP3-44128]

Staining of human liver shows no positivity as expected (negative control).
NF-L Antibody (CL4729) - Azide and BSA Free Immunohistochemistry-Paraffin: NF-L Antibody [NBP3-44128]

Immunohistochemistry-Paraffin: NF-L Antibody [NBP3-44128]

Staining of human cerebral cortex shows strong positivity in neural fibers.
NF-L Antibody (CL4729) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: NF-L Antibody [NBP3-44128]

Immunocytochemistry/ Immunofluorescence: NF-L Antibody [NBP3-44128]

Staining of PC-3 cells using the Anti-NF-L monoclonal antibody).
NF-L Antibody (CL4729) - Azide and BSA Free Immunohistochemistry-Paraffin: NF-L Antibody [NBP3-44128]

Immunohistochemistry-Paraffin: NF-L Antibody [NBP3-44128]

Staining of mouse cerebral cortex shows strong immunoreactivity in neuronal cell bodies and processes.
NF-L Antibody (CL4729) - Azide and BSA Free Western Blot: NF-L Antibody [NBP3-44128]

Western Blot: NF-L Antibody [NBP3-44128]

Analysis in human cerebral cortex tissue.
NF-L Antibody (CL4729) - Azide and BSA Free Immunohistochemistry-Paraffin: NF-L Antibody [NBP3-44128]

Immunohistochemistry-Paraffin: NF-L Antibody [NBP3-44128]

Staining of rat cerebral cortex shows strong immunoreactivity in neuronal cell bodies and processes.
NF-L Antibody (CL4729) - Azide and BSA Free Immunohistochemistry-Paraffin: NF-L Antibody [NBP3-44128]

Immunohistochemistry-Paraffin: NF-L Antibody [NBP3-44128]

Staining of mouse striatum shows strong positivity in neural fibers.
NF-L Antibody (CL4729) - Azide and BSA Free Immunohistochemistry-Paraffin: NF-L Antibody [NBP3-44128]

Immunohistochemistry-Paraffin: NF-L Antibody [NBP3-44128]

Staining of rat brainstem shows strong immunoreactivity in neuronal cell bodies.

Applications for NF-L Antibody (CL4729) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:10000 - 1:20000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF Fixation/Permeabilization: PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NF-L

Neurofilaments are 10nm intermediate filament proteins located in vertebrate neurons, which regulate axonal diameter. They are composed predominantly of the three major neurofilament proteins: NF-Light, NF-Medium and NF-Heavy. NF-L is the light or low molecular weight polypeptide, and it runs on SDS-PAGE gels at about 68kDa, with some size variation in across species.

Antibodies to NF-L may be used to identify NF-L in neurons and to study neuronal processes in tissue sections and/or culture. NF-L antibodies can also be used to study microfilament accumulations seen in many neurological diseases, such as Lou Geri's disease or Alzheimer's disease.

Long Name

Neurofilament Protein, Light Chain

Alternate Names

CMT2E, NEFL, NF68, NFL

Gene Symbol

NEFL

Additional NF-L Products

Product Documents for NF-L Antibody (CL4729) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for NF-L Antibody (CL4729) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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