NF-M Antibody (OTI2G3)

Novus Biologicals | Catalog # NBP2-46139

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2G3
View all NF-M Primary Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 413-773 of human NEFM(NP_005373) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

102.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NF-M Antibody (OTI2G3)

Western Blot: NF-M Antibody (OTI2G3) [NBP2-46139]

Western Blot: NF-M Antibody (OTI2G3) [NBP2-46139]

Western Blot: NF-M Antibody (2G3) [NBP2-46139] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NF-M.
Immunohistochemistry: NF-M Antibody (OTI2G3) [NBP2-46139]

Immunohistochemistry: NF-M Antibody (OTI2G3) [NBP2-46139]

Immunohistochemistry: NF-M Antibody (2G3) [NBP2-46139] - Analysis of Human testicular cancer tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: NF-M Antibody (OTI2G3) [NBP2-46139]

Immunohistochemistry: NF-M Antibody (OTI2G3) [NBP2-46139]

Immunohistochemistry: NF-M Antibody (2G3) [NBP2-46139] - Analysis of Human skin tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: NF-M Antibody (OTI2G3) [NBP2-46139]

Immunohistochemistry: NF-M Antibody (OTI2G3) [NBP2-46139]

Immunohistochemistry: NF-M Antibody (2G3) [NBP2-46139] - Analysis of Human adult heart tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: NF-M Antibody (OTI2G3) [NBP2-46139]

Immunohistochemistry: NF-M Antibody (OTI2G3) [NBP2-46139]

Immunohistochemistry: NF-M Antibody (2G3) [NBP2-46139] - Analysis of Human adult brain tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: NF-M Antibody (OTI2G3) [NBP2-46139]

Immunohistochemistry: NF-M Antibody (OTI2G3) [NBP2-46139]

Immunohistochemistry: NF-M Antibody (2G3) [NBP2-46139] - Analysis of Human melanoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: NF-M Antibody (OTI2G3) [NBP2-46139]

Immunohistochemistry: NF-M Antibody (OTI2G3) [NBP2-46139]

Immunohistochemistry: NF-M Antibody (2G3) [NBP2-46139] - Analysis of Human embryonic liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: NF-M Antibody (OTI2G3) [NBP2-46139]

Immunohistochemistry: NF-M Antibody (OTI2G3) [NBP2-46139]

Immunohistochemistry: NF-M Antibody (2G3) [NBP2-46139] - Analysis of Human muscle tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: NF-M Antibody (OTI2G3) [NBP2-46139]

Immunohistochemistry: NF-M Antibody (OTI2G3) [NBP2-46139]

Immunohistochemistry: NF-M Antibody (2G3) [NBP2-46139] - Analysis of Human embryonic brain cortex tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Immunohistochemistry: NF-M Antibody (OTI2G3) [NBP2-46139]

Immunohistochemistry: NF-M Antibody (OTI2G3) [NBP2-46139]

Immunohistochemistry: NF-M Antibody (2G3) [NBP2-46139] - Analysis of Human embryonic cerebellum tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
NF-M Antibody (OTI2G3)

NF-M Antibody (OTI2G3)[NBP2-46139]

NF-M Antibody (OTI2G3)[NBP2-46139]Simple Western� analysis of lysates (0.2 mg/mL) from HEK293T cell culture using CD63 mouse mAb (SKU: TA803474). EVs were harvested from cell culture media and enriched by polymer-based precipitation. EV contained cell culture media without enrichment was used as controls. The virtual lane view (left) shows the target band corresponding to CD63 (highly glycosylated) at 1:20 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary of EV lysate (Green), cell lysate (Blue) and unenriched media (Grey). The experiment was performed on the Jess Simple Western instrument for ProteinSimple, a BioTechne band, using the 12-230 kDa separation module. *CD63 is highly glycosylated protein, verified by overexpressed lysate tagged with DDK. PNGase F treatment can bring the band to the expected size.

Applications for NF-M Antibody (OTI2G3)

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NF-M

Neurofilaments are 10nm intermediate filament proteins located in vertebrate neurons, which regulate axonal diameter. They are composed predominantly of the three major neurofilament proteins: NF-Light, NF-Medium and NF-Heavy. NF-M is the middle or medium neurofilament subunit and runs on SDS-PAGE gels in the range 145-170kDa, with some size variation in across species.

Antibodies to NF-M may be used to identify NF-M in neurons and to study neuronal processes in tissue sections and/or culture. NF-M can also be useful in studies of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease.

Long Name

Neurofilament Protein, Medium Chain

Alternate Names

NEF3, NEFM, NFM

Gene Symbol

NEFM

Additional NF-M Products

Product Documents for NF-M Antibody (OTI2G3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for NF-M Antibody (OTI2G3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for NF-M Antibody (OTI2G3)

There are currently no reviews for this product. Be the first to review NF-M Antibody (OTI2G3) and earn rewards!

Have you used NF-M Antibody (OTI2G3)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies