NF2/Merlin Antibody (PSH02-52)

Western Blot: NF2/Merlin Antibody (PSH02-52) [NBP3-32590]

Western Blot: NF2/Merlin Antibody (PSH02-52) [NBP3-32590] - Western blot analysis of NF2/Merlin on different lysates with Rabbit anti-NF2/Merlin antibody (NBP3-32590) at 1/2,000 dilution.

Lane 1: PC-3M cell lysate (20 ug/Lane)
Lane 2: HeLa cell lysate (20 ug/Lane)
Lane 3: MDA-MB-468 cell lysate (20 ug/Lane)
Lane 4: SH-SY5Y cell lysate (20 ug/Lane)
Lane 5: HUVEC cell lysate (20 ug/Lane)
Lane 6: HEK-293 cell lysate (20 ug/Lane)
Lane 7: Jurkat cell lysate (20 ug/Lane)
Lane 8: MDA-MB-231 cell lysate (negative) (20 ug/Lane)
Lane 9: COS-1 cell lysate (20 ug/Lane)
Lane 10: Neuro-2a cell lysate (20 ug/Lane)
Lane 11: NIH/3T3 cell lysate (20 ug/Lane)
Lane 12: C6 cell lysate (20 ug/Lane)

Predicted band size: 70 kDa
Observed band size: 70 kDa

Exposure time: 1 minute 41 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32590) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/ Immunofluorescence: NF2/Merlin Antibody (PSH02-52) [NBP3-32590]

Immunocytochemistry/ Immunofluorescence: NF2/Merlin Antibody (PSH02-52) [NBP3-32590] - Immunocytochemistry analysis of HUVEC cells labeling NF2/Merlin with Rabbit anti-NF2/Merlin antibody (NBP3-32590) at 1/100 dilution.

Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NF2/Merlin antibody (NBP3-32590) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.

Flow Cytometry: NF2/Merlin Antibody (PSH02-52) [NBP3-32590]

Flow Cytometry: NF2/Merlin Antibody (PSH02-52) [NBP3-32590] - Flow cytometric analysis of NIH/3T3 cells labeling NF2/Merlin.

Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32590, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).