NFIA Antibody (1E11) - Azide and BSA Free

Novus Biologicals | Catalog # H00004774-M02

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1E11

Format

Azide and BSA Free
View all NFIA Primary Antibodies »

Product Specifications

Immunogen

NFIA (AAH22264, 1 a.a. ~ 498 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MYSPLCLTQDEFHPFIEALLPHVRAFAYTWFNLQARKRKYFKKHEKRMSKEEERAVKDELLSEKPEVKQKWASRLLAKLRKDIRPEYREDFVLTVTGKKPPCCVLSNPDQKGKMRRIDCLRQADKVWRLDLVMVILFKGIPLESTDGERLVKSPQCSNPGLCVQPHHIGVSVKELDLYLAYFVHAADSSQSESPSQPSDADIKDQPENGHLGFQDSFVTSGVFSVTELVRVSQTPIAAGTGPNFSLSDLESSSYYSMSPGAMRRSLPSTSSTSSTKRLKSVEDEMDSPGEEPFYTGQGRSPGSGSQSSGWHEVEPGMPSPTTLKKSEKSGFSSPSPSQTSSLGTAFTQHHRPVITGPRASPHATPSTLHFPTSPIIQQPGPYFSHPAIRYHPQETLKEFVQLVCPDAGQQAGQVGFLNPNGSSQGKVHNPFLPTPMLPPPPPPPMARPVPLPVPDTKPPTTSTEGGAASPTSPILVPGIKVAASHHPPDRPPDPFSTL

Specificity

NFIA - nuclear factor I/A (1E11)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for NFIA Antibody (1E11) - Azide and BSA Free

Western Blot: NFIA Antibody (1E11) [H00004774-M02]

Western Blot: NFIA Antibody (1E11) [H00004774-M02]

Western Blot: NFIA Antibody (1E11) [H00004774-M02] - Analysis of NFIA expression in transfected 293T cell line by NFIA monoclonal antibody (M02), clone 1E11.Lane 1: NFIA transfected lysate (Predicted MW: 54.6 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: NFIA Antibody (1E11) [H00004774-M02]

Immunocytochemistry/ Immunofluorescence: NFIA Antibody (1E11) [H00004774-M02]

Immunocytochemistry/Immunofluorescence: NFIA Antibody (1E11) [H00004774-M02] - Analysis of monoclonal antibody to NFIA on HeLa cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: NFIA Antibody (1E11) [H00004774-M02] - Detection limit for recombinant GST tagged NFIA is approximately 0.3ng/ml as a capture antibody.

Applications for NFIA Antibody (1E11) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NFIA

Nuclear factor I (NFI) proteins constitute a family of dimeric DNA-binding proteins with similar, and possibly identical, DNA-binding specificity. They function as cellular transcription factors and as replication factors for adenovirus DNA replication. Diversity in this protein family is generated by multiple genes, differential splicing, and heterodimerization.[supplied by OMIM]

Alternate Names

CCAAT-box-binding transcription factor, CTF, DKFZp686J23256, FLJ39164, KIAA1439DKFZp434L0422, NF1-A, NF-I/A, NFI-A, NFI-L, nuclear factor 1 A-type, Nuclear factor 1/A, nuclear factor I/ATGGCA-binding protein

Gene Symbol

NFIA

OMIM

600727 (Human)

Additional NFIA Products

Product Documents for NFIA Antibody (1E11) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for NFIA Antibody (1E11) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for NFIA Antibody (1E11) - Azide and BSA Free

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Protocols

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FAQs

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