NFIL3 Antibody
Novus Biologicals | Catalog # NB100-842
![Western Blot: NFIL3 Antibody [NB100-842]](https://resources.rndsystems.com/images/products/NFIL3-Antibody-Western-Blot-NB100-842-img0003.jpg "Western Blot: NFIL3 Antibody [NB100-842]")
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA, Flow Cytometry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Peptide with sequence C-RLIATQPISASDSG corresponding to C-Terminus according to NP_005375.2.
Reactivity Notes
Expected from sequence similarity: Mouse, Rat
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for NFIL3 Antibody
Western Blot: NFIL3 Antibody [NB100-842]
Western Blot: NFIL3 Antibody [NB100-842] - Analysis of NFIL3 in HepG2 lysate using NB100-842 at 0.5ug/ml (RIPA buffer, 20ug total protein per lane). Primary incubated for 1 hour. Detected using chemiluminescence.![Flow Cytometry: NFIL3 Antibody [NB100-842]](https://resources.rndsystems.com/images/products/NFIL3-Antibody-Flow-Cytometry-NB100-842-img0004.jpg "Flow Cytometry: NFIL3 Antibody [NB100-842]")
Flow Cytometry: NFIL3 Antibody [NB100-842]
Flow Cytometry: NFIL3 Antibody [NB100-842] - analysis of paraformaldehyde fixed Human peripheral blood mononuclear cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: UnimmunizedApplications for NFIL3 Antibody
Application
Recommended Usage
Flow Cytometry
0.5-2ug/ml
Peptide ELISA
Detection limit 1:8000
Western Blot
1:1000
Application Notes
Western blot: Approx. 60kDa band observed in nuclear lysates of cell line Jurkat and HeLa (calculated MW of 51.5kDa according to NP_005375.2). The observed molecular weight corresponds to different antibodies from other commercial sources. An additional band of 30kDa was observed in lysates of cell line HeLa, which was completely blocked with the immunizing peptide. Primary incubation 1 hour at room temperature
Flow Cytometry: Flow cytometric analysis of Human peripheral blood mononuclear cells
Flow Cytometry: Flow cytometric analysis of Human peripheral blood mononuclear cells
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: NFIL3/E4BP4
Long Name
Nuclear Factor, Interleukin 3 Regulated
Alternate Names
E4BP4, IL3BP1, NF-IL3A
Gene Symbol
NFIL3
UniProt
Additional NFIL3/E4BP4 Products
Product Documents for NFIL3 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for NFIL3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for NFIL3 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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