NFIX Antibody (HL1269) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-25576
Recombinant Monoclonal Antibody
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Key Product Details
Species Reactivity
Validated:
Mouse, Rat
Predicted:
Human (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1269 expressed in HEK293
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human NFIX. The exact sequence is proprietary.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus (87%).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for NFIX Antibody (HL1269) - Azide and BSA Free
Immunohistochemistry-Paraffin: NFIX Antibody (HL1269) - Azide and BSA Free [NBP3-25576] -
Immunohistochemistry-Paraffin: NFIX Antibody (HL1269) - Azide and BSA Free [NBP3-25576] - NFIX antibody [HL1269] detects NFIX protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissue. NFIX stained by NFIX antibody [HL1269] (NBP3-25576) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 minImmunocytochemistry/Immunofluorescence: NFIX Antibody (HL1269) - Azide and BSA Free [NBP3-25576] -
Immunocytochemistry/Immunofluorescence: NFIX Antibody (HL1269) - Azide and BSA Free [NBP3-25576] - NFIX antibody [HL1269] detects NFIX protein at nucleus by immunofluorescent analysis. Sample: DIV10 rat E18 primary hippocampal neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NFIX stained by NFIX antibody [HL1269] (NBP3-25576) diluted at 1:250. Red: Tau, a cytoskeleton marker, stained by Tau antibody [GT287] diluted at 1:500.Immunohistochemistry-Paraffin: NFIX Antibody (HL1269) - Azide and BSA Free [NBP3-25576] -
Immunohistochemistry-Paraffin: NFIX Antibody (HL1269) - Azide and BSA Free [NBP3-25576] - NFIX antibody [HL1269] detects NFIX protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. NFIX stained by NFIX antibody [HL1269] (NBP3-25576) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 minImmunohistochemistry-Paraffin: NFIX Antibody (HL1269) [NBP3-25576] -
NFIX antibody [HL1269] detects NFIX protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse hippocampus.
NFIX stained by NFIX antibody [HL1269] (NBP3-25576) diluted at 1:800.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: NFIX Antibody (HL1269) [NBP3-25576] -
NFIX antibody [HL1269] detects NFIX protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.
NFIX stained by NFIX antibody [HL1269] (NBP3-25576) diluted at 1:800.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: NFIX Antibody (HL1269) [NBP3-25576] -
NFIX antibody [HL1269] detects NFIX protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse hippocampus.
NFIX stained by NFIX antibody [HL1269] (NBP3-25576) diluted at 1:800.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Applications for NFIX Antibody (HL1269) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:100-1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NFIX
Alternate Names
CTF, NF1ACCAAT-box-binding transcription factor, NF1-X, NF-I/X, NFI-X, nuclear factor 1 X-type, Nuclear factor 1/X, Nuclear factor I/X, nuclear factor I/X (CCAAT-binding transcription factor), TGGCA-binding protein
Gene Symbol
NFIX
Additional NFIX Products
Product Documents for NFIX Antibody (HL1269) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for NFIX Antibody (HL1269) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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