NFkB p105/p50 Antibody (2J10D7) - BSA Free

Novus Biologicals | Catalog # NB100-56583

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat, Rabbit

Cited:

Human, Rat, Rabbit

Applications

Validated:

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Simple Western

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2J10D7

Format

BSA Free
View all NFkB p105/p50 Primary Antibodies »

Product Specifications

Immunogen

A portion of amino acids 150-200 of human NF-kB (p50) was used as the immunogen.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (86%). Rat reactivity reported in scientific literature (PMID:31850807).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for NFkB p105/p50 Antibody (2J10D7) - BSA Free

Western Blot: NFkB p105/p50 Antibody (2J10D7)BSA Free [NB100-56583]

Western Blot: NFkB p105/p50 Antibody (2J10D7)BSA Free [NB100-56583]

Western Blot: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - Analysis of p50 in HeLa lysate in the A) absence and B) presence of immunizing peptide using p50 antibody at 5 ug/ml.
Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody (2J10D7) - BSA Free [NB100-56583]

Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody (2J10D7) - BSA Free [NB100-56583]

Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - IHC-P of rabbit aorta using NB100-56583. Secondary antibody: anti-mouse histofine ( Nisherei Bioscience Inc. ref: 414131F). Development: DAB (Dako ref: K346811-2) and counterstained with Hematoxylin. Submitted via verified customer review
Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) - BSA Free [NB100-56583]

Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) - BSA Free [NB100-56583]

Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on U937 cells with NFkB p105/p50 [2J10D7] Antibody NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.
Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody (2J10D7) - BSA Free [NB100-56583]

Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody (2J10D7) - BSA Free [NB100-56583]

Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - analysis of human breast tumor tissue using an isotype control antibody (top) and p50 antibody (bottom) at 5 ug/ml.
Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) - BSA Free [NB100-56583]

Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) - BSA Free [NB100-56583]

Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on Jurkat cells with NFkB p105/p50 Antibody (2J10D7) NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes. Both antibodies were conjugated to Dylight 650.
Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) - BSA Free [NB100-56583]

Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) - BSA Free [NB100-56583]

Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on HeLa cells with NFkB p105/p50 Antibody (2J10D7) NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes. Both antibodies were conjugated to Dylight 650.
Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) - BSA Free [NB100-56583]

Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) - BSA Free [NB100-56583]

Flow Cytometry: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - An intracellular stain was performed on U2-OS cells with NFkB p105/p50 [2J10D7] Antibody NB100-56583C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.
NFkB p105/p50 Antibody (2J10D7) - BSA Free Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody (2J10D7) - BSA Free [NB100-56583]

Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody (2J10D7) - BSA Free [NB100-56583]

Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody (2J10D7) [NB100-56583] - Analysis of a FFPE tissue section of human lymph node using 1:200 dilution of NFkB p105/p50 (2J10D7) antibody. The staining was developed using HRP labeled anti-mouse secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.

Applications for NFkB p105/p50 Antibody (2J10D7) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100 - 1:200

Immunohistochemistry-Paraffin

1:100 - 1:200

Simple Western

1:10 - 1:250

Western Blot

2-5 ug/ml
Application Notes
See Simple Western Antibody Database for Simple Western validation: Tested in HeLa Lysate, separated by Size, antibody dilution of 1:10, 1:50, 1:250, apparent MW was 56, 126 kDa

Reviewed Applications

Read 1 review rated 5 using NB100-56583 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NFkB p105/p50

Principal Names: Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 (NFKB1); DNA-binding factor KBF1; EBP-1; NFKB-p50; Official Gene Symbol: NFKB1 Gene ID: 4790 (Human) Gene Map Locus: 4q24 (human) NF-kB is a nuclear transcription factor activated by various extra and intracellular stimuli such as cytokines, UV radiation, stress, in injury, and by bacterial and viral products. It is involved in regulation of various cellular events including cell growth, differentiation, proliferation, apoptosis and inflammation. NFKB1 (p50), a 50KDa functional sub-unit of NF-kB, is a member of Rel protein family. It is synthesized as a p105 precursor protein and consists of an N-terminal conserved RHD-region containing nuclear localization signal, DNA-binding and dimerization domains. NFKB1 (p50) forms homodimers or heterodimerizes with p65, forming the functional NF-kB factor. NFKB1 (p50) directs the nuclear translocation of NF-kB and is instrumental in its DNA-bidning. Pathological role of NF-kB has been suggested in AIDS, hematogenic cancer cell metastasis and rheumatoid arthritis.

Alternate Names

DKFZp686C01211, DNA binding factor KBF1, DNA-binding factor KBF1, EBP-1, KBF1, NF-kappaB, NF-kappa-B, NF-kappabeta, NFKB-p105, NFKB-p50, nuclear factor kappa-B DNA binding subunit, nuclear factor NF-kappa-B p105 subunit, nuclear factor NF-kappa-B p50 subunit, nuclear factor of kappa light polypeptide gene enhancer in B-cells 1MGC54151, p105, p50

Entrez Gene IDs

4790 (Human)

Gene Symbol

NFKB1

UniProt

P25942

Additional NFkB p105/p50 Products

Product Documents for NFkB p105/p50 Antibody (2J10D7) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for NFkB p105/p50 Antibody (2J10D7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for NFkB p105/p50 Antibody (2J10D7) - BSA Free

Customer Reviews for NFkB p105/p50 Antibody (2J10D7) - BSA Free (1)

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  • NFkB p105/p50 Antibody (2J10D7)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: aorta artery
    Species: Rabbit
    Verified Customer | Posted 10/02/2017
    Antigen Retrieval: pressure cooker with solution Trilogy TM (Cell Marque ref: 920P-07). Antibody dilution: 0.07ug/mL (diluted 1/4000). Secondary antibody: anti-mouse histofine ( Nisherei Bioscience Inc. ref: 414131F). Development: DAB (Dako ref: K346811-2) and counterstained with Hematoxylin.
    NFkB p105/p50 Antibody (2J10D7) - BSA Free NB100-56583

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Protocols

View specific protocols for NFkB p105/p50 Antibody (2J10D7) - BSA Free (NB100-56583):

Immunohistochemistry-Paraffin Embedded Sections

Antigen Unmasking:
Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes (keep slides in the sodium citrate buffer at all times).

Staining:
1. Wash sections in deionized water three times for 5 minutes each.
2. Wash sections in PBS for 5 minutes.
3. Block each section with 100-400 ul blocking solution (1% BSA in PBS) for 1 hour at room temperature.
4. Remove blocking solution and add 100-400 ul diluted primary antibody. Incubate overnight at 4 C.
5. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
6. Add 100-400 ul HRP polymer conjugated secondary antibody. Incubate 30 minutes at room temperature.
7. Wash sections three times in wash buffer for 5 minutes each.
8. Add 100-400 ul DAB substrate to each section and monitor staining closely.
9. As soon as the sections develop, immerse slides in deionized water.
10. Counterstain sections in hematoxylin.
11. Wash sections in deionized water two times for 5 minutes each.
12. Dehydrate sections.
13. Mount coverslips.


Western Blot Protocol

1. Perform SDS-PAGE on samples to be analyzed, loading 10-25 ug of total protein per lane.
2. Transfer proteins to PVDF membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
3. Stain the membrane with Ponceau S (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
4. Rinse the blot TBS -0.05% Tween 20 (TBST).
5. Block the membrane in 5% Non-fat milk in TBST (blocking buffer) for at least 1 hour.
6. Wash the membrane in TBST three times for 10 minutes each.
7. Dilute primary antibody in blocking buffer and incubate overnight at 4C with gentle rocking.
8. Wash the membrane in TBST three times for 10 minutes each.
9. Incubate the membrane in diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) for 1 hour at room temperature.
10. Wash the blot in TBST three times for 10 minutes each (this step can be repeated as required to reduce background).
11. Apply the detection reagent of choice in accordance with the manufacturer's instructions.

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FAQs

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