NFYB Antibody

Novus Biologicals | Catalog # NBP2-19535

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Zebrafish

Predicted:

Bovine (98%), Porcine (99%), Rhesus Macaque (100%), Xenopus (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all NFYB Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human NFYB. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (92%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NFYB Antibody

Immunohistochemistry-Paraffin: NFYB Antibody [NBP2-19535]

Immunohistochemistry-Paraffin: NFYB Antibody [NBP2-19535]

Immunohistochemistry-Paraffin: NFYB Antibody [NBP2-19535] - Rat stomach. NFYB antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: NFYB Antibody [NBP2-19535]

Immunohistochemistry-Paraffin: NFYB Antibody [NBP2-19535]

Immunohistochemistry-Paraffin: NFYB Antibody [NBP2-19535] - Mouse intestine. NFYB antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
NFYB Antibody

Western Blot: NFYB Antibody [NBP2-19535] -

Western Blot: NFYB Antibody [NBP2-19535] - Various tissue extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NFYB antibody (NBP2-19535) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
NFYB Antibody

Immunohistochemistry: NFYB Antibody [NBP2-19535] -

Immunohistochemistry: NFYB Antibody [NBP2-19535] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using NFYB antibody (NBP2-19535) at 1:300 dilution.
NFYB Antibody

Western Blot: NFYB Antibody [NBP2-19535] -

Western Blot: NFYB Antibody [NBP2-19535] - HeLa whole cell and nuclear extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NFYB antibody (NBP2-19535) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
NFYB Antibody

Western Blot: NFYB Antibody [NBP2-19535] -

Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NFYB antibody (NBP2-19535) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
NFYB Antibody

Immunocytochemistry/ Immunofluorescence: NFYB Antibody [NBP2-19535] -

NFYB antibody detects NFYB protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NFYB protein stained by NFYB antibody (NBP2-19535) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar = 10 um.
NFYB Antibody

Western Blot: NFYB Antibody [NBP2-19535] -

Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with NFYB antibody (NBP2-19535) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
NFYB Antibody

Immunohistochemistry: NFYB Antibody [NBP2-19535] -

Immunohistochemical analysis (whole mount) of zebrafish embryo, using NFYB antibody (NBP2-19535) at 1:200 dilution.
NFYB Antibody

Chromatin Immunoprecipitation: NFYB Antibody [NBP2-19535] -

Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit IgG or anti-NFYB antibody. The precipitated DNA was detected by PCR with primer set targeting to aurora kinase A.
NFYB Antibody

Immunoprecipitation: NFYB Antibody [NBP2-19535] -

NFYB antibody immunoprecipitates NFYB protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 40 ug whole cell lysate/extract of NFYB protein expressing 293T cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of NFYB protein by 2.5 ug of NFYB antibody (NBP2-19535) 10% SDS-PAGE The immunoprecipitated NFYB protein was detected by NFYB antibody (NBP2-19535) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.

Applications for NFYB Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000
Application Notes
Chip-Assay dependent

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NFYB

The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. [provided by RefSeq]

Alternate Names

CAAT box DNA-binding protein subunit B, CBF-A, HAP3CBF-B, NF-YBCCAAT-binding transcription factor subunit A, Nuclear transcription factor Y subunit B, nuclear transcription factor Y subunit beta, nuclear transcription factor Y, beta, Transcription factor NF-Y, B subunit

Gene Symbol

NFYB

UniProt

P25208 (Human)

Additional NFYB Products

Product Documents for NFYB Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for NFYB Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for NFYB Antibody

There are currently no reviews for this product. Be the first to review NFYB Antibody and earn rewards!

Have you used NFYB Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies