NG2/MCSP Antibody (23GB3470)

Novus Biologicals | Catalog # NBP3-51213

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB3470
View all NG2/MCSP Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human NG2/MCSP (Uniprot: Q6UVK1)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NG2/MCSP Antibody (23GB3470)

NG2/MCSP Antibody (23GB3470)

Immunocytochemistry/ Immunofluorescence: NG2/MCSP Antibody (23GB3470)[NBP3-51213] -

Immunocytochemical staining of C2C12 cells with NG2/MCSP (1:1,000). Nuclei were stained blue with DAPI; Chondroitin sulfate proteoglycan 4 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
NG2/MCSP Antibody (23GB3470)

Flow Cytometry: NG2/MCSP Antibody (23GB3470)[NBP3-51213] -

Validation of NG2/MCSP knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with NG2/MCSP antibody (1:2,000) and analyzed using BD flow cytometer.
NG2/MCSP Antibody (23GB3470)

Flow Cytometry: NG2/MCSP Antibody (23GB3470)[NBP3-51213] -

Flow cytometric analysis of NG2/MCSPexpression in C2C12 cells using NG2/MCSP antibody (1:2,000). Green, isotype control; red, Chondroitin sulfate proteoglycan 4.
NG2/MCSP Antibody (23GB3470)

Western Blot: NG2/MCSP Antibody (23GB3470)[NBP3-51213] -

Western blotting analysis using NG2/MCSP antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for NG2/MCSP Antibody (23GB3470)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NG2/MCSP

Proteoglycans (PGs) are a family of proteins composed of different polypeptide chains containing glycosaminoglycan (GAG) modifications. They vary in their cellular locations and have diverse structure and functions. NG2 is a proteoglycan found in the nervous system, comprising a large integral membrane PG with a core protein of 300 kDa and at least one covalently attached chondroitin sulfate (CS) GAG chain. In the CNS this protein is expressed mainly on the surfaces of developing and adult oligodendrocyte precursor cells, but is also associated with developing chondrocytes, cardiomyocytes, pericytes and several human tumours.

Long Name

Chondroitin Sulfate Proteoglycan NG2/Melanoma Associated Chondroitin Sulfate Proteoglycan

Alternate Names

AN2, CSPG4, HMW-MAA, MCSP, MCSPG, MEL-CSPG

Gene Symbol

CSPG4

Additional NG2/MCSP Products

Product Documents for NG2/MCSP Antibody (23GB3470)

Certificate of Analysis

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Product Specific Notices for NG2/MCSP Antibody (23GB3470)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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