NHE8 Antibody (7A11) [Allophycocyanin]

Novus Biologicals | Catalog # NB110-62091APC

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG Clone # 7A11
View all NHE8 Primary Antibodies »

Product Specifications

Immunogen

A fusion protein containing the C-terminal 89 residues of human NHE8. [Swiss-Prot# Q9Y2E8]

Reactivity Notes

Human, mouse and rat.

Localization

Golgi apparatus membrane; multi-pass membrane.

Specificity

This is specific for the 85 kDa mature glycosylated form of the protein. It does not recognize the 55 kDa immature form.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Theoretical MW

80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications for NHE8 Antibody (7A11) [Allophycocyanin]

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This NHE8 antibody is useful for Western blot analysis where a band is seen at ~80 kDa. Please note that these samples should not be boiled.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: NHE8

NHE8, also known as SLC9A8, is an integral transmembrane proteins that exchange extracellular Na+ for intracellular H+. NHE8 is expressed in the kidney, where it may contribute to apical membrane ion transport physiology. NHE8 is highly expressed in the proximal tubules in the outer stripe of the outer medulla with significant but lower expression in the proximal tubules in the cortex. Sodium-Hydrogen ion Exchangers (NHEs) are a growing family of transmembrane proteins, and NHE8 antibodies are useful tools for research on NaH ion exchange.

Long Name

Sodium/hydrogen exchanger 8

Alternate Names

KIAA0939, NHE-8, SLC9A8

Gene Symbol

SLC9A8

Additional NHE8 Products

Product Documents for NHE8 Antibody (7A11) [Allophycocyanin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for NHE8 Antibody (7A11) [Allophycocyanin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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